PMID: 6986909Mar 4, 1980Paper

Escherichia coli phosphoenolpyruvate-dependent phosphotransferase system. Evidence that the dimer is the active form of enzyme I

Biochemistry
O MissetG T Robillard

Abstract

In vitro kinetic measurements have been performed by using purified HPr, EI, and a membrane fraction of EII from the Escherichia coli phosphoenolypyruvate-dependent sugar transport system. These measurements reveal very large lag times in the formation of methyl alpha-glucoside phosphate which are a function of the EI and the EII concentrations. The lag times decrease with increasing concentrations of EI but they increase with increasing concentrations of EII. When EI, together with Mg2+ and phosphoenolpyruvate, is preincubated at 37 degrees C before starting the kinetic measurements, the lag time can be decreased or eliminated. We have shown that the process responsible for the lag time is the activation of EI by dimerization which is influenced by Mg2+ and phosphoenolpyruvate.

References

Dec 14, 1976·Biochimica Et Biophysica Acta·P W Postma, S Roseman
Jan 1, 1977·Current Topics in Microbiology and Immunology·W Hengstenberg
Nov 1, 1976·Proceedings of the Society for Experimental Biology and Medicine·L C Garg, C Y Chiou
Jul 1, 1969·The Journal of General Physiology·S Roseman

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Citations

Oct 1, 1992·Biophysical Chemistry·F ChauvinL Brand
Dec 13, 2006·Microbiology and Molecular Biology Reviews : MMBR·Josef DeutscherPieter W Postma
Feb 14, 2002·Archives of Biochemistry and Biophysics·Ann Ginsburg, Alan Peterkofsky
Oct 21, 2006·Proceedings of the National Academy of Sciences of the United States of America·Alexey TeplyakovOsnat Herzberg
Sep 1, 2012·Protein Science : a Publication of the Protein Society·Young-Joo Yun, Jeong-Yong Suh
Sep 5, 2013·Protein Science : a Publication of the Protein Society·Young-Joo YunJeong-Yong Suh
Dec 14, 2001·The Journal of Biological Chemistry·Luis Fernando Garcia-AllesBernhard Erni
Jul 27, 2006·The Journal of Biological Chemistry·Joséa MárquezKlaus Scheffzek
Sep 1, 1985·Microbiological Reviews·P W Postma, J W Lengeler
Sep 1, 1993·Microbiological Reviews·P W PostmaG R Jacobson

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