Escherichia coli ribosomal protein S1 unfolds structured mRNAs onto the ribosome for active translation initiation

PLoS Biology
Mélodie DuvalStefano Marzi

Abstract

Regulation of translation initiation is well appropriate to adapt cell growth in response to stress and environmental changes. Many bacterial mRNAs adopt structures in their 5' untranslated regions that modulate the accessibility of the 30S ribosomal subunit. Structured mRNAs interact with the 30S in a two-step process where the docking of a folded mRNA precedes an accommodation step. Here, we used a combination of experimental approaches in vitro (kinetic of mRNA unfolding and binding experiments to analyze mRNA-protein or mRNA-ribosome complexes, toeprinting assays to follow the formation of ribosomal initiation complexes) and in vivo (genetic) to monitor the action of ribosomal protein S1 on the initiation of structured and regulated mRNAs. We demonstrate that r-protein S1 endows the 30S with an RNA chaperone activity that is essential for the docking and the unfolding of structured mRNAs, and for the correct positioning of the initiation codon inside the decoding channel. The first three OB-fold domains of S1 retain all its activities (mRNA and 30S binding, RNA melting activity) on the 30S subunit. S1 is not required for all mRNAs and acts differently on mRNAs according to the signals present at their 5' ends. This work sho...Continue Reading

References

Aug 5, 1978·Journal of Molecular Biology·J O ThomasW Szer
Jun 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A KolbW Szer
Dec 14, 1977·Molecular & General Genetics : MGG·A R Subramanian, J van Duin
Jun 15, 1978·Journal of Molecular Biology·M Laughrea, P B Moore
Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·D G BearH F Noller
Aug 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·A E Dahlberg, J E Dahlberg
Jan 11, 1991·Nucleic Acids Research·I V BoniN V Tzareva
Jan 1, 1988·Methods in Enzymology·D HartzL Gold
Jan 1, 1988·Annual Review of Biochemistry·L Gold
May 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·C PhilippeC Ehresmann
Oct 1, 1996·Current Opinion in Structural Biology·D P Millar
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·J M Jean, K B Hall
Aug 21, 2001·Cell·G Z YusupovaH F Noller
Oct 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·J SenguptaJ Frank
Mar 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Eric Massé, Susan Gottesman
Mar 5, 2003·Trends in Genetics : TIG·Pascale Romby, Mathias Springer
Apr 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·K KobayashiN Ogasawara
Aug 12, 2003·Journal of Molecular Biology·Maarten H de Smit, Jan van Duin
Sep 17, 2003·Genes & Development·Eric MasséSusan Gottesman
Mar 1, 1965·Journal of Molecular Biology·M REVEL, H H HIATT
Jan 24, 2004·The EMBO Journal·Thomas A Geissmann, Danièle Touati
Sep 2, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kathleen E McGinness, Robert T Sauer
Jan 18, 2005·Cell·Seyedtaghi TakyarHarry F Noller
Apr 8, 2006·Molecular Cell·Sean M Studer, Simpson Joseph
Oct 20, 2006·Nature·Gulnara YusupovaMarat Yusupov

❮ Previous
Next ❯

Citations

Jan 24, 2014·Nucleic Acids Research·Heena KhatterBruno P Klaholz
Aug 6, 2014·Nucleic Acids Research·Julie-Anna M Benjamin, Eric Massé
Aug 15, 2014·Nucleic Acids Research·Daijiro TakeshitaKozo Tomita
Sep 4, 2014·International Journal of Molecular Sciences·Kozo Tomita
Feb 6, 2016·Wiley Interdisciplinary Reviews. RNA·Michael J Ellis, David B Haniford
Nov 19, 2015·Nucleic Acids Research·Heidi GytzCharlotte R Knudsen
May 21, 2016·Journal of the American Chemical Society·Amin Espah Borujeni, Howard M Salis
Dec 29, 2016·Methods : a Companion to Methods in Enzymology·Mélodie DuvalStefano Marzi
Mar 21, 2017·Biochimica Et Biophysica Acta. General Subjects·Justin R J Vigar, Hans-Joachim Wieden
Dec 17, 2014·Nucleic Acids Research·Konstantin ByrgazovIsabella Moll
Oct 14, 2017·ELife·Gabriel DemoAndrei A Korostelev
Jul 12, 2018·Nature Reviews. Microbiology·Erik Holmqvist, Jörg Vogel
Aug 4, 2018·Wiley Interdisciplinary Reviews. RNA·Radoslaw Pluta, Manuel Espinosa
Apr 18, 2018·Cold Spring Harbor Perspectives in Biology·Marina V Rodnina
Apr 12, 2017·Journal of Bacteriology·Angel A AguirreMarilis V Marques
Jan 23, 2019·Journal of Biochemistry·Juan C Cifuentes-GochesJavier Hernández-Sánchez
Nov 18, 2018·The Journal of Antimicrobial Chemotherapy·Miranda E PittMatthew A Cooper
Jul 8, 2018·Molecular Systems Biology·André MateusMikhail M Savitski
Nov 25, 2019·Wiley Interdisciplinary Reviews. RNA·Claude Chiaruttini, Maude Guillier
Dec 4, 2019·The Plant Journal : for Cell and Molecular Biology·Kenneth P WatkinsAlice Barkan
Dec 14, 2019·Nucleic Acids Research·Paul E LundNils G Walter
Jun 26, 2020·PLoS Genetics·Lucia GrengaJacob George Malone
Jun 16, 2016·Wiley Interdisciplinary Reviews. RNA·Michelle M Meyer
Apr 2, 2017·G3 : Genes - Genomes - Genetics·Akram AbolbaghaeiXuhua Xia
May 21, 2016·Protein Science : a Publication of the Protein Society·Marina V Rodnina
Jul 20, 2019·Proceedings of the National Academy of Sciences of the United States of America·Cédric RomillyE Gerhart H Wagner
Apr 1, 2020·Molecular Biotechnology·Anna NizkorodovaBulat Iskakov
May 20, 2020·Genome Research·Syue-Ting KuoHsin-Hung David Chou
Jul 28, 2020·International Journal of Molecular Sciences·Sergei Y GrishinOxana V Galzitskaya

❮ Previous
Next ❯

Methods Mentioned

BETA
toeprinting
filter binding
surface plasmon resonance
size exclusion chromatography
NMR
PCR
electrophoresis
equilibrium binding

Software Mentioned

Vienna package
graphpad PRISM
Prism Graphpad
RNAcofold
SAFA

Related Concepts

Related Feeds

Barrel cortex

Here is the latest research on barrel cortex, a region of somatosensory and motor corticies in the brain, which are used by animals that rely on whiskers for world exploration.