Escherichia coli RNA polymerase core and holoenzyme structures

The EMBO Journal
R D FinnM van Heel

Abstract

Multisubunit RNA polymerase is an essential enzyme for regulated gene expression. Here we report two Escherichia coli RNA polymerase structures: an 11.0 A structure of the core RNA polymerase and a 9.5 A structure of the sigma(70) holoenzyme. Both structures were obtained by cryo-electron microscopy and angular reconstitution. Core RNA polymerase exists in an open conformation. Extensive conformational changes occur between the core and the holoenzyme forms of the RNA polymerase, which are largely associated with movements in ss'. All common RNA polymerase subunits (alpha(2), ss, ss') could be localized in both structures, thus suggesting the position of sigma(70) in the holoenzyme.

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Citations

Apr 7, 2004·Journal of Structural Biology·J R PlaisierJ P Abrahams
Sep 6, 2002·Biochimica Et Biophysica Acta·Michael S Kobor, Jack Greenblatt
Jan 5, 2002·The Biochemical Journal·Jöelle RoubyJean-Paul Leonetti
Mar 29, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Frédéric CollandAnnie Kolb
Apr 2, 2002·Journal of Biomolecular Structure & Dynamics·I S MasulisO N Ozoline
Apr 4, 2006·Biophysical Journal·Oana CobanG Ulrich Nienhaus
Jul 24, 2008·Journal of Molecular Biology·Yara X MejiaCarlos Bustamante
Oct 14, 2017·ELife·Gabriel DemoAndrei A Korostelev
Oct 20, 2009·Gene Regulation and Systems Biology·Sousuke Imamura, Munehiko Asayama
Jan 26, 2021·Analytical Chemistry·Kevin Jeanne Dit FouqueFrancisco Fernandez-Lima
Feb 16, 2021·ACS Nano·Nesrine AissaouiGaetan Bellot
Aug 30, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Evgeniy V DubrovinTilman E Schäffer

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