PMID: 11531998Sep 5, 2001Paper

Escherichia coli RNA polymerase subunit omega and its N-terminal domain bind full-length beta' to facilitate incorporation into the alpha2beta subassembly

European Journal of Biochemistry
P GhoshDipankar Chatterji


The omega subunit of Escherichia coli RNA polymerase, consisting of 90 amino acids, is present in stoichiometric amounts per molecule of core RNA polymerase (alpha2betabeta'). The presence of omega is necessary to restore denatured RNA polymerase in vitro to its fully functional form, and, in an omega-less strain of E. coli, GroEL appears to substitute for omega in the maturation of RNA polymerase. The X-ray structure of Thermus aquaticus core RNA polymerase suggests that two regions of omega latch on to beta' at its N-terminus and C-terminus. We show here that omega binds only the intact beta' subunit and not the beta' N-terminal domain or beta' C-terminal domain, implying that omega binding requires both these regions of beta'. We further show that omega can prevent the aggregation of beta' during its renaturation in vitro and that a V8-protease-resistant 52-amino-acid-long N-terminal domain of omega is sufficient for binding and renaturation of beta'. CD and functional assays show that this N-terminal fragment retains the structure of native omega and is able to enhance the reconstitution of core RNA polymerase. Reconstitution of core RNA polymerase from its individual subunits proceeds according to the steps alpha + alpha -...Continue Reading


Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·C O PaboM Ptashne
Jun 1, 1992·Journal of Bacteriology·M LonettoC A Gross
Nov 1, 1991·Journal of Virological Methods·W A TatarowiczK D Thompson
Sep 1, 1990·Protein Expression and Purification·D R Gentry, R R Burgess
May 18, 1973·Science·R R Porter
May 15, 1981·Biochemical and Biophysical Research Communications·W Stender
Jan 1, 1996·Methods in Enzymology·N Fujita, A Ishihama
Jan 1, 1997·Folding & Design·J E CoyleS E Radford
Oct 29, 1999·European Journal of Biochemistry·K MukherjeeD Chatterji
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·P GoloubinoffB Bukau
Jan 26, 2000·Nature·W A HouryF U Hartl
Feb 1, 2000·The Journal of Biological Chemistry·A KatayamaA Ishihama
Feb 7, 2001·Proceedings of the National Academy of Sciences of the United States of America·L MinakhinK Severinov
Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R T Ogata, W Gilbert

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