PMID: 11531998Sep 5, 2001Paper

Escherichia coli RNA polymerase subunit omega and its N-terminal domain bind full-length beta' to facilitate incorporation into the alpha2beta subassembly

European Journal of Biochemistry
P GhoshDipankar Chatterji

Abstract

The omega subunit of Escherichia coli RNA polymerase, consisting of 90 amino acids, is present in stoichiometric amounts per molecule of core RNA polymerase (alpha2betabeta'). The presence of omega is necessary to restore denatured RNA polymerase in vitro to its fully functional form, and, in an omega-less strain of E. coli, GroEL appears to substitute for omega in the maturation of RNA polymerase. The X-ray structure of Thermus aquaticus core RNA polymerase suggests that two regions of omega latch on to beta' at its N-terminus and C-terminus. We show here that omega binds only the intact beta' subunit and not the beta' N-terminal domain or beta' C-terminal domain, implying that omega binding requires both these regions of beta'. We further show that omega can prevent the aggregation of beta' during its renaturation in vitro and that a V8-protease-resistant 52-amino-acid-long N-terminal domain of omega is sufficient for binding and renaturation of beta'. CD and functional assays show that this N-terminal fragment retains the structure of native omega and is able to enhance the reconstitution of core RNA polymerase. Reconstitution of core RNA polymerase from its individual subunits proceeds according to the steps alpha + alpha -...Continue Reading

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Citations

May 10, 2008·Journal of Proteome Research·Oleg PaliyA Keith Dunker
Jun 29, 2002·Applied and Environmental Microbiology·Paula M PeriagoJeroen A Wouters
Nov 10, 2012·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Akira Ishihama
Jun 5, 2015·Scientific Reports·Johanna DrögemüllerPaul Rösch
Nov 13, 2015·Scientific Reports·Johanna DrögemüllerStefan H Knauer
Apr 17, 2015·FEMS Microbiology Reviews·Andy Weiss, Lindsey N Shaw
Mar 16, 2017·Biophysical Journal·Debipreeta BhowmikDipankar Chatterji
Mar 16, 2018·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Neerupma BhardwajDipankar Chatterji
Jan 31, 2014·Nucleic Acids Research·Liisa GunneliusTaina Tyystjärvi
Jun 10, 2003·The Journal of Biological Chemistry·Cynthia L RichardRobert G Kranz
Sep 14, 2020·Molecular Microbiology·Juha KurkelaTaina Tyystjärvi
Dec 24, 2018·Biochemical Society Transactions·David Forrest
Mar 7, 2019·Biochemical Society Transactions·Amber Riaz-Bradley
Jul 12, 2013·The Journal of Biological Chemistry·Paramita SarkarDipankar Chatterji
Nov 27, 2020·Biomolecules·Unnatiben Rajeshbhai PatelDipankar Chatterji
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Jun 1, 2021·Frontiers in Molecular Biosciences·Tomasz W Turowski, Magdalena Boguta

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