PMID: 3911944Dec 1, 1985Paper

Escherichia coli S-adenosylhomocysteine/5'-methylthioadenosine nucleosidase. Purification, substrate specificity and mechanism of action

The Biochemical Journal
F Della RagioneA E Pegg

Abstract

S-Adenosylhomocysteine/5'-methylthioadenosine nucleosidase (EC 3.2.2.9) was purified to homogeneity from Escherichia coli to a final specific activity of 373 mumol of 5'-methylthioadenosine cleaved/min per mg of protein. Affinity chromatography on S-formycinylhomocysteine-Sepharose is the key step of the purification procedure. The enzyme, responsible for the cleavage of the glycosidic bond of both S-adenosylhomocysteine and 5'-methylthioadenosine, was partially characterized. The apparent Km for 5'-methylthioadenosine is 0.4 microM, and that for S-adenosylhomocysteine is 4.3 microM. The maximal rate of cleavage of S-adenosylhomocysteine is approx. 40% of that of 5'-methylthioadenosine. Some 25 analogues of the two naturally occurring thioethers were studied as potential substrates or inhibitors of the enzyme. Except for the analogues modified in the 5'-position of the ribose moiety or the 2-position of the purine ring, none of the compounds tested was effective as a substrate. Moreover, 5'-methylthioformycin, 5'-chloroformycin, S-formycinylhomocysteine, 5'-methylthiotubercidin and S-tubercidinylhomocysteine were powerful inhibitors of the enzyme activity. The results obtained allow the hypothesis of a mechanism of enzymic cata...Continue Reading

Citations

Mar 31, 2009·FEBS Letters·Martin R ChallandPeter L Roach
Oct 13, 2015·Chembiochem : a European Journal of Chemical Biology·Daniel ShinRajesh Nagarajan
Jun 18, 2010·Journal of Structural Biology·Karen K W SiuP Lynne Howell
Nov 10, 2007·Phytochemistry·Tomás Rezanka, Karel Sigler
May 20, 2004·Bioorganic & Medicinal Chemistry Letters·Martina E TedderKraig M Yager
Mar 18, 2008·Journal of Molecular Biology·Karen K W SiuP Lynne Howell
Oct 23, 2012·ACS Chemical Biology·Richard BaranTrent R Northen
Aug 8, 2001·Molecular Microbiology·Stephan SchauderBonnie L Bassler
Apr 4, 2017·Chembiochem : a European Journal of Chemical Biology·Joanna C SadlerGlenn A Burley
Jul 16, 2008·Biomedical Microdevices·Xiaolong LuoGary W Rubloff
Jan 6, 2010·Chembiochem : a European Journal of Chemical Biology·Christian DalhoffElmar Weinhold
Nov 20, 2016·Proceedings of the National Academy of Sciences of the United States of America·Michael T BancoDonald R Ronning
Jun 12, 2002·Journal of Bacteriology·Anne L Beeston, Michael G Surette

Related Concepts

Pfs protein, E coli
Chromatography, Affinity
SDS-PAGE
Alkalescens-Dispar Group
Nucleosidases
Substrate Specificity

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Lipidomics & Rhinovirus Infection

Lipidomics can be used to examine the lipid species involved with pathogenic conditions, such as viral associated inflammation. Discovered the latest research on Lipidomics & Rhinovirus Infection.

Glut1 Deficiency

Glut1 deficiency, an autosomal dominant, genetic metabolic disorder associated with a deficiency of GLUT1, the protein that transports glucose across the blood brain barrier, is characterized by mental and motor developmental delays and infantile seizures. Follow the latest research on Glut1 deficiency with this feed.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Laryngeal Neoplasms

Laryngeal Neoplasms occur in the Larynx and are typically associated with smoking and alcohol consumption. Discover the latest research on Laryngeal Neoplasms here.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.