Escherichia coli ST8196 is a novel, locally evolved, and extensively drug resistant pathogenic lineage within the ST131 clonal complex.

Emerging Microbes & Infections
Priyanka HastakPiklu Roy Chowdhury

Abstract

The H30Rx subclade of Escherichia coli ST131 is a clinically important, globally dispersed pathogenic lineage that typically displays resistance to fluoroquinolones and extended spectrum β-lactams. Isolates EC233 and EC234, variants of ST131-H30Rx with a novel sequence type (ST) 8196, isolated from unrelated patients presenting with bacteraemia at a Sydney Hospital in 2014 are characterised here. EC233 and EC234 are phylogroup B2, serotype O25:H4A, and resistant to ampicillin, amoxicillin, cefoxitin, ceftazidime, ceftriaxone, ciprofloxacin, norfloxacin and gentamicin and are likely clonal. Both harbour an IncFII_2 plasmid (pSPRC_Ec234-FII) that carries most of the resistance genes on an IS26 associated translocatable unit, two small plasmids and a novel IncI1 plasmid (pSPRC_Ec234-I). SNP-based phylogenetic analysis of the core genome of representatives within the ST131 clonal complex places both isolates in a subclade with three clinical Australian ST131-H30Rx clade-C isolates. A MrBayes phylogeny analysis of EC233 and EC234 indicates ST8196 share a most recent common ancestor with ST131-H30Rx strain EC70 isolated from the same hospital in 2013. Our study identified genomic hallmarks that define the ST131-H30Rx subclade in the ...Continue Reading

References

Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Dec 25, 2003·Antimicrobial Agents and Chemotherapy·R Bonnet
Jul 28, 2006·Antimicrobial Agents and Chemotherapy·A RobicsekD C Hooper
Apr 25, 2007·Infection and Immunity·Nicola HoldenDavid L Gally
Feb 1, 2011·Bioinformatics·Mitchell J SullivanScott A Beatson
Jul 29, 2011·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A-P MagiorakosD L Monnet
Aug 10, 2011·BMC Genomics·Nabil-Fareed AlikhanScott A Beatson
Feb 24, 2012·Systematic Biology·Fredrik RonquistJohn P Huelsenbeck
Mar 15, 2012·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Laurent PoirelPatrice Nordmann
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Nicola K PettyScott A Beatson
May 29, 2014·Antimicrobial Agents and Chemotherapy·Ritu Banerjee, James R Johnson
Sep 10, 2014·Gut Pathogens·Aaron E DarlingSteven P Djordjevic
Jan 18, 2015·Advances in Applied Microbiology·Amy J MathersJohann D D Pitout
Jun 3, 2015·Open Forum Infectious Diseases·Mary J BurgessRitu Banerjee
Jul 23, 2015·Bioinformatics·Andrew J PageJulian Parkhill
Dec 10, 2015·MBio·Christopher J Harmer, Ruth M Hall
Mar 24, 2016·MBio·Nicole StoesserUNKNOWN Modernizing Medical Microbiology Informatics Group (MMMIG)
Mar 28, 2017·F1000Research·Johann D D Pitout, Rebekah DeVinney
May 26, 2017·The Journal of Antimicrobial Chemotherapy·Edward R BevanPeter M Hawkey
Jun 9, 2017·PLoS Computational Biology·Ryan R WickKathryn E Holt
Jan 15, 2019·Microbiology Resource Announcements·Lauren BrooksMark Sistrom
Apr 24, 2019·Antimicrobial Agents and Chemotherapy·Daniel J DiekemaRonald N Jones
Jul 13, 2019·The Journal of Antimicrobial Chemotherapy·Luana C MeloJean-Yves Madec
Aug 15, 2019·Nature Communications·Brian M FordeScott A Beatson

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Datasets Mentioned

BETA
SAMN10187599
SRR10126950

Methods Mentioned

BETA
Assay

Software Mentioned

BLAST
Adobe PhotoShop CS6
HMM
BLASTn
MrBayes
BLASTp
island
RAxML
Unicycler hybrid genome assembler
Bayes

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