Escherichia coli translation initiation factor 3 discriminates the initiation codon in vivo

Molecular Microbiology
Jacqueline K SussmanRobert W Simons


In a genetic selection designed to isolate Escherichia coli mutations that increase expression of the IS 10 transposase gene (tnp), we unexpectedly obtained viable mutants defective in translation initiation factor 3 (IF3). Several lines of evidence led us to conclude that transposase expression, per se, was not increased. Rather, these mutations appear to increase expression of the tnp'-'lacZ gene fusions used in this screen, by increasing translation initiation at downstream, atypical initiation codons. To test this hypothesis we undertook a systematic analysis of start codon requirements and measured the effects of IF3 mutations on initiation from various start codons. Beginning with an efficient translation initiation site, we varied the AUG start codon to all possible codons that differed from AUG by one nucleotide. These potential start codons fall into distinct classes with regard to translation efficiency in vivo: Class I codons (AUG, GUG, and UUG) support efficient translation; Class IIA codons (CUG, AUU, AUC, AUA, and ACG) support translation at levels only 1-3% that of AUG; and Class IIB codons (AGG and AAG) permit levels of translation too low for reliable quantification, importantly, the IF3 mutations had no effect...Continue Reading


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Aug 14, 2001·The EMBO Journal·D PetrelliClaudio O Gualerzi
May 24, 2003·Cold Spring Harbor Symposia on Quantitative Biology·Claudio O GualerziC L Pon
May 24, 2003·Cold Spring Harbor Symposia on Quantitative Biology·A BashanAda Yonath
Mar 5, 2009·Antimicrobial Agents and Chemotherapy·Minghua WangMinggui Wang
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Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·J P McCutcheonJ Frank
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Jan 8, 2008·Journal of Molecular Biology·Ashesh A SaraiyaPhilip R Cunningham
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May 14, 2005·Journal of Molecular Biology·Dale LindsleyAshley Fontelera
Jul 23, 2011·Microbiology·Mollie W JewettPatricia A Rosa
Apr 8, 2006·Molecular Cell·Sean M Studer, Simpson Joseph
May 18, 1999·Journal of Molecular Biology·C SacerdotM Springer
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