In a genetic selection designed to isolate Escherichia coli mutations that increase expression of the IS 10 transposase gene (tnp), we unexpectedly obtained viable mutants defective in translation initiation factor 3 (IF3). Several lines of evidence led us to conclude that transposase expression, per se, was not increased. Rather, these mutations appear to increase expression of the tnp'-'lacZ gene fusions used in this screen, by increasing translation initiation at downstream, atypical initiation codons. To test this hypothesis we undertook a systematic analysis of start codon requirements and measured the effects of IF3 mutations on initiation from various start codons. Beginning with an efficient translation initiation site, we varied the AUG start codon to all possible codons that differed from AUG by one nucleotide. These potential start codons fall into distinct classes with regard to translation efficiency in vivo: Class I codons (AUG, GUG, and UUG) support efficient translation; Class IIA codons (CUG, AUU, AUC, AUA, and ACG) support translation at levels only 1-3% that of AUG; and Class IIB codons (AGG and AAG) permit levels of translation too low for reliable quantification, importantly, the IF3 mutations had no effect...Continue Reading
Identification of the start codon for sphS encoding the phosphate-sensing histidine kinase in Synechocystis sp. PCC 6803
Identification of the ribosome binding sites of translation initiation factor IF3 by multidimensional heteronuclear NMR spectroscopy
The interdomain linker of Escherichia coli initiation factor IF3: a possible trigger of translation initiation specificity
Expression of the Escherichia coli pcnB gene is translationally limited using an inefficient start codon: a second chromosomal example of translation initiated at AUU
The activation-induced cytidine deaminase (AID) efficiently targets DNA in nucleosomes but only during transcription
Selective stimulation of translation of leaderless mRNA by initiation factor 2: evolutionary implications for translation
Role of the N- and C-terminal extensions on the activity of mammalian mitochondrial translational initiation factor 3
Fine-tuning of the ribosomal decoding center by conserved methyl-modifications in the Escherichia coli 16S rRNA
The antibiotic Furvina® targets the P-site of 30S ribosomal subunits and inhibits translation initiation displaying start codon bias
Whole proteome analysis of post-translational modifications: applications of mass-spectrometry for proteogenomic annotation
High-resolution structures of ribosomal subunits: initiation, inhibition, and conformational variability
Molecular characterisation of the pifC gene encoding translation initiation factor 3, which is required for normal photosynthetic complex formation in Rhodobacter sphaeroides NCIB 8253
New plasmid-mediated quinolone resistance gene, qnrC, found in a clinical isolate of Proteus mirabilis
Depletion of free 30S ribosomal subunits in Escherichia coli by expression of RNA containing Shine-Dalgarno-like sequences
The gas vesicle gene cluster from Microcystis aeruginosa and DNA rearrangements that lead to loss of cell buoyancy
Functional elements in initiation factors 1, 1A, and 2β discriminate against poor AUG context and non-AUG start codons
An mRNA sequence derived from a programmed frameshifting signal decreases codon discrimination during translation initiation
In vivo involvement of mutated initiation factor IF1 in gene expression control at the translational level
Translation initiation factor 3 regulates switching between different modes of ribosomal subunit joining
A single mutation in the IF3 N-terminal domain perturbs the fidelity of translation initiation at three levels
Identification and role of functionally important motifs in the 970 loop of Escherichia coli 16S ribosomal RNA
Identification of an alternative translation initiation site for the Pantoea ananatis lycopene cyclase (crtY) gene in E. coli and its evolutionary conservation
Reduced expression of antigenic proteins MPB70 and MPB83 in Mycobacterium bovis BCG strains due to a start codon mutation in sigK
Control of translation initiation involves a factor-induced rearrangement of helix 44 of 16S ribosomal RNA
Overexpression of RbfA in the absence of the KsgA checkpoint results in impaired translation initiation
Multiple ways to regulate translation initiation in bacteria: Mechanisms, regulatory circuits, dynamics
The emerging role of rectified thermal fluctuations in initiator aa-tRNA- and start codon selection during translation initiation
The translational fidelity function of IF3 during transition from the 30 S initiation complex to the 70 S initiation complex
mua-6, a gene required for tissue integrity in Caenorhabditis elegans, encodes a cytoplasmic intermediate filament
The search and its outcome: high-resolution structures of ribosomal particles from mesophilic, thermophilic, and halophilic bacteria at various functional states
The Effect of an Alternate Start Codon on Heterologous Expression of a PhoA Fusion Protein in Mycoplasma gallisepticum
Initiation factor 3-induced structural changes in the 30 S ribosomal subunit and in complexes containing tRNA(f)(Met) and mRNA
An AUG initiation codon, not codon-anticodon complementarity, is required for the translation of unleadered mRNA in Escherichia coli
Identification of mammalian mitochondrial translational initiation factor 3 and examination of its role in initiation complex formation with natural mRNAs.
Contributions of the N- and C-Terminal Domains of Initiation Factor 3 to Its Functions in the Fidelity of Initiation and Antiassociation of the Ribosomal Subunits
Translational Control of the SigR-Directed Oxidative Stress Response in Streptomyces via IF3-Mediated Repression of a Noncanonical GTC Start Codon
Translation initiation rate determines the impact of ribosome stalling on bacterial protein synthesis.
Reinitiation of protein synthesis in Escherichia coli can be induced by mRNA cis-elements unrelated to canonical translation initiation signals
Codon-Anticodon Recognition in the Bacillus subtilis glyQS T Box Riboswitch: RNA-DEPENDENT CODON SELECTION OUTSIDE THE RIBOSOME.
Combinatorial modulation of initial codons for improved zeaxanthin synthetic pathway efficiency in Escherichia coli
Accumulation of pre-apocytochrome f in a Synechocystis sp. PCC 6803 mutant impaired in cytochrome c maturation.
Regulation of the activity of chloroplast translational initiation factor 3 by NH2- and COOH-terminal extensions.
Polyamine enhancement of the synthesis of adenylate cyclase at the translational level and the consequential stimulation of the synthesis of the RNA polymerase sigma 28 subunit
Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq - ryhB encodes the regulatory RNA RyhB and a peptide, RyhP
Differing isoforms of the cobalamin binding photoreceptor AerR oppositely regulate photosystem expression
Start-Stop Assembly: a functionally scarless DNA assembly system optimized for metabolic engineering
A Novel pH-Regulated, Unusual 603 bp Overlapping Protein Coding Gene pop Is Encoded Antisense to ompA in Escherichia coli O157:H7 (EHEC)
Construction and initial characterization of Escherichia coli strains with few or no intact chromosomal rRNA operons
The Oenococcus oeni clpX homologue is a heat shock gene preferentially expressed in exponential growth phase
Structural basis of sequestration of the anti-Shine-Dalgarno sequence in the Bacteroidetes ribosome.
Genetic and immunologic analyses of PlpE, a lipoprotein important in complement-mediated killing of Pasteurella haemolytica serotype 1
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