Escherichia coli with a Tunable Point Mutation Rate for Evolution Experiments.

G3 : Genes - Genomes - Genetics
Nicholas A Sherer, Thomas E Kuhlman


The mutation rate and mutations' effects on fitness are crucial to evolution. Mutation rates are under selection due to linkage between mutation rate modifiers and mutations' effects on fitness. The linkage between a higher mutation rate and more beneficial mutations selects for higher mutation rates, while the linkage between a higher mutation rate and more deleterious mutations selects for lower mutation rates. The net direction of selection on mutations rates depends on the fitness landscape, and a great deal of work has elucidated the fitness landscapes of mutations. However, tests of the effect of varying a mutation rate on evolution in a single organism in a single environment have been difficult. This has been studied using strains of antimutators and mutators, but these strains may differ in additional ways and typically do not allow for continuous variation of the mutation rate. To help investigate the effects of the mutation rate on evolution, we have genetically engineered a strain of Escherichia coli with a point mutation rate that can be smoothly varied over two orders of magnitude. We did this by engineering a strain with inducible control of the mismatch repair proteins MutH and MutL. We used this strain in an ap...Continue Reading


Nov 1, 1994·International Journal of Food Microbiology·J Baranyi, T A Roberts
Dec 28, 1999·Methods : a Companion to Methods in Enzymology·W A Rosche, P L Foster
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Sep 25, 2003·Antimicrobial Agents and Chemotherapy·Patricia Komp LindgrenDiarmaid Hughes
Jun 24, 2004·Evolution; International Journal of Organic Evolution·Paulo R A Campos, Viviane M de Oliveira
Mar 24, 2007·Molecular Biology and Evolution·Jonathan P Bollback, John P Huelsenbeck
Dec 25, 2007·Cell Research·Guo-Min Li
Mar 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·Dmitry NevozhayGábor Balázsi
May 18, 2010·Mutation Research·Martin G Marinus
Aug 21, 2010·Journal of Nucleic Acids·Kenji Fukui
Mar 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Benjamin H GoodMichael M Desai
Dec 28, 2012·Proceedings of the National Academy of Sciences of the United States of America·Richard A Neher, Oskar Hallatschek
Oct 18, 2013·Nature Communications·Howard H Chou, Jay D Keasling
Jan 1, 2014·BMC Systems Biology·Daniel MadarUri Alon
May 20, 2014·Methods in Molecular Biology·Daniel E Deatherage, Jeffrey E Barrick
Jan 13, 2015·Proceedings of the National Academy of Sciences of the United States of America·Brian Charlesworth
Sep 4, 2015·PloS One·Huseyin TasThomas E Kuhlman
Apr 7, 2016·Scientific Reports·Arvi Jõers, Tanel Tenson
Aug 2, 2016·Nature·Olivier TenaillonRichard E Lenski
Sep 21, 2016·Genetics·Benjamin H Good, Michael M Desai
Jun 1, 1998·Evolution; International Journal of Organic Evolution·Michael LynchMatthew Giorgianni
Oct 27, 2017·Proceedings of the National Academy of Sciences of the United States of America·Alejandro CouceOlivier Tenaillon
Mar 29, 2018·Science·Lydia RobertMarina Elez
Apr 28, 2018·PLoS Genetics·Kathleen SprouffskeAndreas Wagner

❮ Previous
Next ❯


❮ Previous
Next ❯

Datasets Mentioned


Methods Mentioned


Software Mentioned

AATI Fragment Analyzer

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.