Escherichia coli with a Tunable Point Mutation Rate for Evolution Experiments.

G3 : Genes - Genomes - Genetics
Nicholas A Sherer, Thomas E Kuhlman

Abstract

The mutation rate and mutations' effects on fitness are crucial to evolution. Mutation rates are under selection due to linkage between mutation rate modifiers and mutations' effects on fitness. The linkage between a higher mutation rate and more beneficial mutations selects for higher mutation rates, while the linkage between a higher mutation rate and more deleterious mutations selects for lower mutation rates. The net direction of selection on mutations rates depends on the fitness landscape, and a great deal of work has elucidated the fitness landscapes of mutations. However, tests of the effect of varying a mutation rate on evolution in a single organism in a single environment have been difficult. This has been studied using strains of antimutators and mutators, but these strains may differ in additional ways and typically do not allow for continuous variation of the mutation rate. To help investigate the effects of the mutation rate on evolution, we have genetically engineered a strain of Escherichia coli with a point mutation rate that can be smoothly varied over two orders of magnitude. We did this by engineering a strain with inducible control of the mismatch repair proteins MutH and MutL. We used this strain in an ap...Continue Reading

References

Nov 1, 1994·International Journal of Food Microbiology·J Baranyi, T A Roberts
Dec 28, 1999·Methods : a Companion to Methods in Enzymology·W A Rosche, P L Foster
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Sep 25, 2003·Antimicrobial Agents and Chemotherapy·Patricia Komp LindgrenDiarmaid Hughes
Jun 24, 2004·Evolution; International Journal of Organic Evolution·Paulo R A Campos, Viviane M de Oliveira
Mar 24, 2007·Molecular Biology and Evolution·Jonathan P Bollback, John P Huelsenbeck
Dec 25, 2007·Cell Research·Guo-Min Li
Mar 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·Dmitry NevozhayGábor Balázsi
May 18, 2010·Mutation Research·Martin G Marinus
Aug 21, 2010·Journal of Nucleic Acids·Kenji Fukui
Mar 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Benjamin H GoodMichael M Desai
Dec 28, 2012·Proceedings of the National Academy of Sciences of the United States of America·Richard A Neher, Oskar Hallatschek
Oct 18, 2013·Nature Communications·Howard H Chou, Jay D Keasling
Jan 1, 2014·BMC Systems Biology·Daniel MadarUri Alon
May 20, 2014·Methods in Molecular Biology·Daniel E Deatherage, Jeffrey E Barrick
Jan 13, 2015·Proceedings of the National Academy of Sciences of the United States of America·Brian Charlesworth
Sep 4, 2015·PloS One·Huseyin TasThomas E Kuhlman
Apr 7, 2016·Scientific Reports·Arvi Jõers, Tanel Tenson
Aug 2, 2016·Nature·Olivier TenaillonRichard E Lenski
Sep 21, 2016·Genetics·Benjamin H Good, Michael M Desai
Jun 1, 1998·Evolution; International Journal of Organic Evolution·Michael LynchMatthew Giorgianni
Oct 27, 2017·Proceedings of the National Academy of Sciences of the United States of America·Alejandro CouceOlivier Tenaillon
Mar 29, 2018·Science·Lydia RobertMarina Elez
Apr 28, 2018·PLoS Genetics·Kathleen SprouffskeAndreas Wagner

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Datasets Mentioned

BETA
PRJNA589707

Methods Mentioned

BETA
PCR

Software Mentioned

gdtools
AATI Fragment Analyzer
GENEWIZ
breseq

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