Jul 10, 2014

Escherichia [corrected] coli ribose binding protein based bioreporters revisited

Scientific Reports
Artur ReimerJan Roelof van der Meer

Abstract

Bioreporter bacteria, i.e., strains engineered to respond to chemical exposure by production of reporter proteins, have attracted wide interest because of their potential to offer cheap and simple alternative analytics for specified compounds or conditions. Bioreporter construction has mostly exploited the natural variation of sensory proteins, but it has been proposed that computational design of new substrate binding properties could lead to completely novel detection specificities at very low affinities. Here we reconstruct a bioreporter system based on the native Escherichia coli ribose binding protein RbsB and one of its computationally designed variants, reported to be capable of binding 2,4,6-trinitrotoluene (TNT). Our results show in vivo reporter induction at 50 nM ribose, and a 125 nM affinity constant for in vitro ribose binding to RbsB. In contrast, the purified published TNT-binding variant did not bind TNT nor did TNT cause induction of the E. coli reporter system.

  • References34
  • Citations5

References

Mentioned in this Paper

Escherichia
Binding Protein
Ribose
Escherichia Infection
Trinitrotoluene
Escherichia coli
Entamoeba coli
In Vitro [Publication Type]

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