DOI: 10.1101/002790Feb 18, 2014Paper

ESCRT-0 is not required for ectopic Notch activation and tumor suppression in Drosophila

BioRxiv : the Preprint Server for Biology
Emiliana TognonThomas Vaccari

Abstract

Multivesicular endosome (MVE) sorting depends on proteins of the Endosomal Sorting Complex Required for Transport (ESCRT) family. These are organized in four complexes (ESCRT-0, -I, -II, -III) that act in a sequential fashion to deliver ubiquitylated cargoes into the internal luminal vesicles (ILVs) of the MVE. Drosophila genes encoding ESCRT-I, -II, -III components function in sorting signaling receptors, including Notch and the JAK/STAT signaling receptor Domeless. Loss of ESCRT-I, -II, -III in Drosophila epithelia causes altered signaling and cell polarity, suggesting that ESCRTs genes are tumor suppressors. However, the nature of the tumor suppressive function of ESCRTs, and whether tumor suppression is linked to receptor sorting is unclear. Unexpectedly, a null mutant in Hrs, encoding one of components of the ESCRT-0 complex, which acts upstream of ESCRT-I, -II, -III in MVE sorting is dispensable for tumor suppression. Here, we report that two Drosophila epithelia lacking activity of Stam , the other known components of the ESCRT-0 complex, or of both Hrs and Stam fail to degrade signaling receptors. However, mutant tissue surprisingly maintains normal apico-basal polarity and proliferation control and does not display ect...Continue Reading

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