ESCRT-I core and ESCRT-II GLUE domain structures reveal role for GLUE in linking to ESCRT-I and membranes.

Cell
Hsiangling TeoRoger L Williams

Abstract

ESCRT complexes form the main machinery driving protein sorting from endosomes to lysosomes. Currently, the picture regarding assembly of ESCRTs on endosomes is incomplete. The structure of the conserved heterotrimeric ESCRT-I core presented here shows a fan-like arrangement of three helical hairpins, each corresponding to a different subunit. Vps23/Tsg101 is the central hairpin sandwiched between the other subunits, explaining the critical role of its "steadiness box" in the stability of ESCRT-I. We show that yeast ESCRT-I links directly to ESCRT-II, through a tight interaction of Vps28 (ESCRT-I) with the yeast-specific zinc-finger insertion within the GLUE domain of Vps36 (ESCRT-II). The crystal structure of the GLUE domain missing this insertion reveals it is a split PH domain, with a noncanonical lipid binding pocket that binds PtdIns3P. The simultaneous and reinforcing interactions of ESCRT-II GLUE domain with membranes, ESCRT-I, and ubiquitin are critical for ubiquitinated cargo progression from early to late endosomes.

References

Jan 16, 1999·Current Biology : CB·M Fernandez-BorjaJ Neefjes
Sep 6, 2000·The EMBO Journal·D J GilloolyH Stenmark
Sep 13, 2000·Molecular Cell·S E LietzkeD G Lambright
Jan 13, 2001·The Journal of Biological Chemistry·N Bishop, P Woodman
Jan 5, 2002·The Journal of Cell Biology·C E FutterC R Hopkins
Dec 4, 2002·Nature Reviews. Molecular Cell Biology·David J KatzmannScott D Emr
Jan 29, 2003·The Journal of Biological Chemistry·Kristi G BacheHarald Stenmark
Mar 29, 2003·Journal of Virology·Juan Martin-SerranoPaul D Bieniasz
Aug 2, 2003·Current Opinion in Cell Biology·Camilla RaiborgHarald Stenmark
Aug 6, 2003·The Journal of Cell Biology·David J KatzmannScott D Emr
Aug 6, 2003·The Journal of Cell Biology·Owen PornillosWesley I Sundquist
Aug 6, 2003·The Journal of Cell Biology·Kristi G BacheHarald Stenmark
Sep 25, 2003·Cell·Bettina StrackHeinrich G Göttlinger
Sep 25, 2003·Cell·Uta K von SchwedlerWesley I Sundquist
Oct 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Juan Martin-SerranoAnton Yaravoy
Oct 23, 2003·Annual Review of Cell and Developmental Biology·Linda Hicke, Rebecca Dunn
Oct 30, 2003·The Biochemical Journal·Jeremy W PeckPeter D Burbelo
Mar 19, 2004·The EMBO Journal·Steven L AlamWesley I Sundquist
Apr 1, 2004·Molecular Cell·Wesley I SundquistChristopher P Hill
Jun 26, 2004·The Journal of Biological Chemistry·Melissa D StuchellWesley I Sundquist
Aug 25, 2004·The Journal of Cell Biology·Natalie Luhtala, Greg Odorizzi
Aug 27, 2004·Nature·Aitor HierroJames H Hurley
Nov 30, 2004·Virus Research·Dimiter G Demirov, Eric O Freed
Dec 22, 2004·The Journal of Biological Chemistry·Rosa Puertollano
Mar 10, 2005·The Journal of Biological Chemistry·Thomas SlagsvoldHarald Stenmark
May 26, 2005·Biochimica Et Biophysica Acta·Katherine Bowers, Tom H Stevens

❮ Previous
Next ❯

Citations

Jun 26, 2007·Pflügers Archiv : European journal of physiology·Avia Rosenhouse-Dantsker, Diomedes E Logothetis
Mar 29, 2011·Plant Molecular Biology·Mojgan ShahriariSwen Schellmann
Nov 17, 2007·Cell Host & Microbe·Winfried Weissenhorn, Heinrich Göttlinger
Nov 10, 2009·Nature Cell Biology·Paramasivam NatarajanTodd R Graham
Dec 24, 2011·Nature Cell Biology·Tor Erik RustenHarald Stenmark
Mar 24, 2007·Nature Reviews. Immunology·Hiroshi Takayanagi
Apr 24, 2007·Nature Reviews. Molecular Cell Biology·Roger L Williams, Sylvie Urbé
Jan 25, 2008·Nature Reviews. Molecular Cell Biology·Mark A Lemmon
Jul 1, 2010·Nature Reviews. Molecular Cell Biology·James H Hurley, Phyllis I Hanson
Aug 19, 2007·Molecular BioSystems·Daniel A BlasioleAlan D Attie
Jan 20, 2007·Biochemical Society Symposium·Mark A Lemmon
May 21, 2011·Proceedings of the National Academy of Sciences of the United States of America·Evzen BouraJames H Hurley
Oct 10, 2012·Proceedings of the National Academy of Sciences of the United States of America·Michael A HaddersJuan Martin-Serrano
Dec 17, 2009·The Journal of Biological Chemistry·Andrew M EllisdonMurray Stewart
Aug 30, 2008·The Journal of Biological Chemistry·Claudia WalliserTom D Bunney
Jul 22, 2008·The Journal of Biological Chemistry·Wenyu WenMingjie Zhang
Jul 21, 2006·Molecular Biology of the Cell·Wenyu WenDavid K Banfield
Dec 8, 2006·Molecular Biology of the Cell·Andrea J OestreichDavid J Katzmann
May 4, 2007·Molecular Biology of the Cell·Ivana De DomenicoJerry Kaplan
Dec 14, 2007·Molecular Biology of the Cell·Bethan McDonald, Juan Martin-Serrano
Aug 25, 2012·Molecular Biology of the Cell·Joëlle MorvanSylvie Friant
Feb 3, 2007·Current Opinion in Gastroenterology·Erik Madsen, Jonathan D Gitlin
Sep 16, 2006·Journal of Virology·Charles LangelierWesley I Sundquist
May 18, 2007·Annual Review of Cell and Developmental Biology·Robert C Piper, David J Katzmann
Jan 12, 2013·Retrovirology·Bo Meng, Andrew Ml Lever
Jun 19, 2009·Journal of Cell Science·Bethan McDonald, Juan Martin-Serrano
Nov 15, 2013·Molecular Biology of the Cell·Alexander R KolbJeffrey L Brodsky
Mar 27, 2013·Annual Review of Biochemistry·John McCulloughWesley I Sundquist
Oct 24, 2006·Nature Structural & Molecular Biology·Steven L AlamWesley I Sundquist
Oct 24, 2006·Nature Structural & Molecular Biology·Satoshi HiranoSoichi Wakatsuki
Feb 27, 2009·Chemical Reviews·Brian A DaviesDavid J Katzmann
Jul 12, 2014·Cells·Alice ZuinBernat Crosas
Jan 23, 2008·The Journal of Cell Biology·Phyllis I HansonJohn E Heuser
Dec 6, 2006·The Journal of Cell Biology·Tony ChuScott D Emr
Dec 3, 2014·Current Opinion in Microbiology·Miguel A PeñalvaHerbert N Arst
May 17, 2011·Current Opinion in Cell Biology·Markus Babst
Oct 30, 2010·Trends in Biochemical Sciences·Suman PeelWinfried Weissenhorn
Dec 9, 2014·Insect Biochemistry and Molecular Biology·Zhaofei Li, Gary Blissard

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autophagosome

An autophagosome is the formation of double-membrane vesicles that involve numerous proteins and cytoplasmic components. These double-membrane vesicles are then terminated at the lysosome where they are degraded. Discover the latest research on autophagosomes here.

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.

Autophagosome

An autophagosome is the formation of double-membrane vesicles that involve numerous proteins and cytoplasmic components. These double-membrane vesicles are then terminated at the lysosome where they are degraded. Discover the latest research on autophagosomes here.