ESCRT-III mediates budding across the inner nuclear membrane and regulates its integrity

Nature Communications
Jun AriiYasushi Kawaguchi

Abstract

Vesicle-mediated nucleocytoplasmic transport is a nuclear pore-independent mechanism for the nuclear export of macromolecular complexes, but the molecular basis for this transport remains largely unknown. Here we show that endosomal sorting complex required for transport-III (ESCRT-III) is recruited to the inner nuclear membrane (INM) during the nuclear export of herpes simplex virus 1 (HSV-1). Scission during HSV-1 budding through the INM is prevented by depletion of ESCRT-III proteins. Interestingly, in uninfected human cells, the depletion of ESCRT-III proteins induces aberrant INM proliferation. Our results show that HSV-1 expropriates the ESCRT-III machinery in infected cells for scission of the INM to produce vesicles containing progeny virus nucleocapsids. In uninfected cells, ESCRT-III regulates INM integrity by downregulating excess INM.

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Citations

Apr 19, 2019·Journal of Virology·Jenna Barnes, Duncan W Wilson
Feb 8, 2020·The Journal of Cell Biology·Brandt WareckiWilliam Sullivan
Mar 21, 2020·Cells·Julia E HölperThomas C Mettenleiter
Nov 11, 2019·Nature Reviews. Molecular Cell Biology·Marina VietriHarald Stenmark
Jul 1, 2020·Nature Cell Biology·Marina VietriCoen Campsteijn
Sep 20, 2020·Viruses·Shilpa GuptaSaveez Saffarian
Jun 25, 2020·Viruses·Anita F MeierMichael Seyffert
Jun 13, 2020·Frontiers in Cell and Developmental Biology·Christos DogrammatzisMaria Kalamvoki
May 21, 2020·Science China. Life Sciences·Xia LiXian-En Zhang
Aug 23, 2020·International Journal of Molecular Sciences·Imran Ahmad, Duncan W Wilson
Sep 30, 2020·Seminars in Cell & Developmental Biology·Yael RadenMotti Gerlic
Nov 4, 2020·Scientific Reports·Jun AriiYasushi Kawaguchi
Nov 4, 2020·Proceedings of the National Academy of Sciences of the United States of America·Susanne LieseAndreas Carlson
Dec 31, 2020·Viruses·Christos DogrammatzisMaria Kalamvoki
Mar 6, 2021·Journal of Cell Science·Miguel Hernandez-GonzalezMichael Way
Mar 7, 2021·Cells·Arianna CalistriCristina Parolin
Apr 13, 2021·Frontiers in Molecular Biosciences·Raeesah HayatudinNurolaini Kifli
Nov 7, 2020·The International Journal of Biochemistry & Cell Biology·Freya MolenberghsWinnok H De Vos
Aug 28, 2021·International Journal of Molecular Sciences·Yichen JuLiying Zhang
Jan 22, 2022·The Journal of Cell Biology·Keisuke MochidaHitoshi Nakatogawa

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Methods Mentioned

BETA
confocal microscopy
super-resolution microscopy
confocal
electron microscopy
FCS
PCR
antisense oligonucleotides
transfection
electrophoresis

Software Mentioned

GraphPad Prism
Elements
ZEN2
ImageJ
GraphPad
NIS

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