Esculetin Attenuates Th2 and Th17 Responses in an Ovalbumin-Induced Asthmatic Mouse Model

Inflammation
Long Hongyan

Abstract

The purpose of the current study was to investigate the anti-asthmatic effect of esculetin (ES) and explore its potential mechanism with a mouse model of allergic asthma. A total number of 50 mice were randomly assigned to five groups: control, model, dexamethasone (Dex, 2 mg/kg), and ES (20 mg/kg, 40 mg/kg). Mouse asthma model was developed with the sensitization and challenge of ovalbumin (OVA). The levels of IgE in serum, eosinophilia infiltration, Th2/Th17 cytokines, Th17 cell frequency, histological condition, and the protein expressions of RORγt, GATA3 were detected. Our study demonstrated that ES inhibited, OVA-induced eosinophil count, interleukin-4 (IL-4), IL-5, IL-13, and IL-17A levels were recovered in bronchoalveolar lavage fluid. Flow cytometry (FCM) studies revealed that ES substantially inhibited Th17 cells' percentage. Western blot study also indicated that ES downregulated RORγt and GATA3 expressions. Meanwhile, ES had beneficial effects on the histological alteration. These findings suggested that ES might effectively ameliorate the progression of asthma and could be used as a therapy for patients with allergic asthma.

References

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Citations

Feb 9, 2017·Canadian Journal of Physiology and Pharmacology·Juan LiHongxin Wang
Aug 23, 2019·Phytotherapy Research : PTR·Chundan XieYingying Wang
Jun 19, 2019·Clinical and Experimental Pharmacology & Physiology·Bin SunMin Xu
Jan 2, 2019·International Journal of Molecular Sciences·Franco FratiSusanna Esposito
May 8, 2020·Oxidative Medicine and Cellular Longevity·Emad H M HassaneinAyman M Mahmoud
Mar 7, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marwa KerbouaJesus Miguel Lopez Rodilla
Jun 5, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Shi WuJinhao Zeng
Nov 23, 2021·Phytotherapy Research : PTR·Linlin ZhangXiaofang Li

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