ESIPT-based fluorescence probe for the rapid detection of hypochlorite (HOCl/ClO- )

Chemical Communications : Chem Comm
Luling WuTony D James

Abstract

ESIPT-based fluorescence probes are emerging as an attractive tool for the detection of biologically relevant analytes owing to their unique photophysical properties. In this work, we have developed an ESIPT-based fluorescence probe (TCBT-OMe) for the detection of HClO/ClO- through the attachment of a bioorthogonal dimethylthiocarbamate linker. TCBT-OMe was shown to rapidly detect HClO/ClO- (<10 s) at biologically relevant concentrations (LoD = 0.16 nM) and have an excellent selectivity towards others ROS/RNS and amino acids. Therefore, TCBT-OMe was tested in live cells and was successfully shown to be able to detect endogenous and exogenous HClO/ClO- in HeLa cells. Additionally, TCBT-OMe acts as a dual input logic gate for Hg2+ and H2O2. Interestingly, Hg2+ alone gradually causes a fluorescence response but requires >30 min to produce a fluorescence response. Test strips containing TCBT-OMe were prepared and were demonstrated as an effective way to detect HClO/ClO- in water. Furthermore, TCBT-OMe was shown to detect exogenously added HClO/ClO- in three different water samples with little interference thus demonstrating the effectiveness as a method for the detection of HClO/ClO- in drinking water samples.

References

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Citations

Sep 25, 2018·Chemical Communications : Chem Comm·Luling WuTony D James
Oct 27, 2018·Chemical Society Reviews·Adam C SedgwickJuyoung Yoon
Jul 28, 2019·Chemistry, an Asian Journal·Tesla YudhistiraDavid G Churchill
Sep 19, 2019·Chemistry, an Asian Journal·Jiamin WangJianhong Wang
Jul 16, 2020·Chemical Communications : Chem Comm·Janeala J MorsbyBradley D Smith
May 27, 2021·Organic & Biomolecular Chemistry·Abhishek SainiSonu Kumar
Jun 25, 2021·Chemical Science·Xue TianTony D James
Dec 24, 2019·The Journal of Physical Chemistry. a·Yusuke YonedaHiroshi Miyasaka

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Methods Mentioned

BETA
Fluorescence
PMA

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