PMID: 9537553Apr 16, 1998Paper

Esophageal/pyloric ligation enhances development of the murine fetal stomach in organ culture

Journal of Pediatric Surgery
S E ZgleszewskiM R Chinoy

Abstract

The authors hypothesized that increased intraluminal pressure in the fetal stomach would enhance development in a murine organ culture model. Gestation day 14 (Gd14) fetal stomachs from time-dated pregnant CD-1 mice (term, 20 days) were maintained in organ culture for 7 days. Some stomachs were ligated at the gastroesophageal (GE) and pyloroduodenal (PD) junctions. Others were left unligated. Gd14, Gd16, and Gd18 stomachs were taken as well to compare organogenesis in vivo. Tissues were processed for histological, morphometric, and immunohistochemical analysis, as well as total protein and DNA determination. The ligated stomachs were visibly distended compared with unligated stomachs in organ culture after 7 days. The length and width of the 7-day in vitro ligated stomachs were significantly increased compared with unligated (2.97+/-0.04 mm v 2.48+/-0.05 mm and 2.14+/-0.04 mm v 1.57+/-0.08 mm, respectively, P < .05). Mucosal epithelial cells showed nuclear polarization, and there was a distinct outer muscle layer in the ligated stomachs, but not in the unligated stomachs, which demonstrated pseudostratified epithelial cells in the mucosa. The ligated stomachs had increased in mucosal thickness compared with unligated (31.4+/-1....Continue Reading

References

Feb 1, 1990·Journal of Pediatric Surgery·A L VelascoM M Ziegler
Feb 1, 1989·Journal of Pediatric Gastroenterology and Nutrition·D G JacobsT Heim
Apr 2, 1987·Nature·R BravoH Macdonald-Bravo
Apr 1, 1986·The Journal of Surgical Research·S J MulvihillH T Debas
Dec 1, 1985·Journal of Pediatric Surgery·S J MulvihillE W Fonkalsrud
Apr 1, 1982·The Journal of Physiology·A DembińskiM Polański
Oct 1, 1984·The Journal of Clinical Endocrinology and Metabolism·T J MerimeeJ E Tyson
Nov 1, 1993·Journal of Pediatric Surgery·T L BuchmillerE W Fonkalsrud
Jul 1, 1996·Journal of Pediatric Surgery·C J BlewettR E Cilley
Aug 1, 1997·Journal of Pediatric Surgery·S E ZgleszewskiM R Chinoy

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Citations

Jul 28, 2001·Development, Growth & Differentiation·K AngioiA Duprez
Apr 5, 2000·Journal of Pediatric Gastroenterology and Nutrition·F MuhaleK Angioï

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