EspJ of enteropathogenic and enterohaemorrhagic Escherichia coli inhibits opsono-phagocytosis.

Cellular Microbiology
Oliver MarchèsEmmanuelle Caron

Abstract

A key strategy in microbial pathogenesis is the subversion of the first line of cellular immune defences presented by professional phagocytes. Enteropathogenic and enterohaemorrhagic Escherichia coli (EPEC and EHEC respectively) remain extracellular while colonizing the gut mucosa by attaching and effacing mechanism. EPEC use the type three secretion system effector protein EspF to prevent their own uptake into macrophages. EPEC can also block in trans the internalization of IgG-opsonized particles. In this study, we show that EspJ is the type three secretion system effector protein responsible for trans-inhibition of macrophage opsono-phagocytosis by both EPEC and EHEC. While EspF plays no role in trans-inhibition of opsono-phagocytosis, espJ mutants of EPEC or EHEC are unable to block uptake of opsonized sheep red blood cells (RBC), a phenotype that is rescued upon complementation with the espJ gene. Importantly, ectopic expression of EspJ(EHEC) in phagocytes is sufficient to inhibit internalization of both IgG- and C3bi-opsonized RBC. These results suggest that EspJ targets a basic mechanism common to these two unrelated phagocytic receptors. Moreover, EspF and EspJ target independent aspects of the phagocytic function of ma...Continue Reading

References

Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A E JerseJ B Kaper
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·K G JarvisJ B Kaper
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·T K McDanielJ B Kaper
Dec 1, 1996·Infection and Immunity·H Ochman, E A Groisman
Jan 23, 1999·Infection and Immunity·D L GoosneyB B Finlay
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Aug 26, 2000·Journal of Bacteriology·E Schlosser-SilvermanS Altuvia
Mar 10, 2001·The Journal of Clinical Investigation·B P McNamaraG Hecht
Apr 12, 2001·Cellular Microbiology·J K CraneM S Donnenberg
Apr 16, 2002·Molecular Biology of the Cell·Jayesh C PatelEmmanuelle Caron
Jul 17, 2003·Cellular Microbiology·Scott R Waterman, David W Holden
Mar 26, 2004·Nature Reviews. Microbiology·James B KaperHarry L Mobley
Oct 2, 2004·Current Biology : CB·Brian K CoombesB Brett Finlay
Oct 8, 2004·The EMBO Journal·Virginie BraunFlorence Niedergang
Oct 11, 2005·Annual Review of Cell and Developmental Biology·Aron B Jaffe, Alan Hall
Jan 13, 2006·Current Opinion in Microbiology·Emmanuelle CaronGad Frankel

❮ Previous
Next ❯

Citations

Nov 21, 2009·The Journal of Biological Chemistry·Eleanor GrovesEmmanuelle Caron
May 11, 2013·Science·Leigh A BaxtMarcia B Goldberg
Aug 18, 2009·Applied and Environmental Microbiology·István TóthBéla Nagy
Aug 4, 2010·Infection and Immunity·Ashleigh HolmesPaul Dean
Dec 21, 2010·PloS One·Ana Blanco-ToribioLuis Ángel Fernández
Dec 31, 2009·PLoS Pathogens·Xiaofei GaoPhilip R Hardwidge
Aug 31, 2011·PLoS Pathogens·Maria FookesNicholas R Thomson
Sep 14, 2011·Annual Review of Pathology·Ronald S FlannaganSergio Grinstein
Apr 15, 2011·Molecular Microbiology·Alexander R C WongElizabeth L Hartland
Feb 12, 2009·FEMS Microbiology Reviews·Roberto M La RagioneAndrew D Wales
May 5, 2012·Gut Microbes·Abigail ClementsGad Frankel
Jan 16, 2009·Current Opinion in Microbiology·Paul Dean, Brendan Kenny
Jul 5, 2016·Biomicrofluidics·Jie-Pan Shen, Chia-Fu Chou
Nov 29, 2016·Annual Review of Genetics·Jaclyn S PearsonElizabeth L Hartland
Jan 16, 2014·MBio·Charley C Gruber, Vanessa Sperandio
Oct 5, 2013·Clinical Microbiology Reviews·Matthew A CroxenB Brett Finlay
Dec 8, 2009·Nature Reviews. Microbiology·Matthew A Croxen, B Brett Finlay
Aug 5, 2014·Nature Reviews. Microbiology·James W CollinsGad Frankel
Aug 22, 2017·Reports on Progress in Physics·David M Richards, Robert G Endres
Sep 9, 2020·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Rigoberto Hernández-CastroUlises Garza-Ramos
Aug 19, 2010·Cellular Microbiology·M Alexander Schmidt

❮ Previous
Next ❯

Methods Mentioned

BETA
PMA
confocal microscopy
PCR
FCS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.