EspP, a novel extracellular serine protease of enterohaemorrhagic Escherichia coli O157:H7 cleaves human coagulation factor V

Molecular Microbiology
Werner BrunderHelge Karch

Abstract

In this study, we identified and characterized a novel secreted protein, the extracellular serine protease EspP, which is encoded by the large plasmid of enterohaemorrhagic Escherichia coli (EHEC) O157:H7. The corresponding espP gene consists of a 3900 bp open reading frame that is able to encode a 1300-amino-acid protein. EspP is synthesized as a large precursor which is then processed at the N- and C-termini during secretion. It can be grouped into the autotransporter protein family. The deduced amino acid sequence of EspP showed homology to several secreted or surface-exposed proteins of pathogenic bacteria, in particular EspC of enteropathogenic E. coli and IgA1 proteases from Neisseria spp. and Haemophilus influenzae. Hybridization experiments and immunoblot analysis of clinical EHEC isolates showed that EspP is widespread among EHEC of the serogroup O157 and that it also exists in serogroup 026. A specific immune response against EspP was detected in sera from patients suffering from EHEC infections. Functional analysis showed that EspP is a protease capable of cleaving pepsin A and human coagulation factor V. Degradation of factor V could contribute to the mucosal haemorrhage observed in patients with haemorrhagic colitis.

Citations

Jan 1, 2013·Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz·H KarchA Mellmann
Nov 30, 2000·Current Infectious Disease Reports·J L Jaeger, D W Acheson
Apr 22, 2011·Applied Biochemistry and Biotechnology·Mohamed BennaniHamid Amarouch
Apr 10, 2013·Research in Microbiology·Jan GrijpstraJan Tommassen
Jan 22, 2004·FEMS Microbiology Letters·Nick J ParhamIan R Henderson
Jul 14, 1999·Diagnostic Microbiology and Infectious Disease·H KarchH Schmidt
Oct 21, 1998·Trends in Microbiology·I R HendersonJ P Nataro
Mar 6, 1999·Current Opinion in Microbiology·J B Kaper
Nov 13, 2007·Nature Structural & Molecular Biology·Travis J BarnardSusan K Buchanan
Aug 7, 2010·The Journal of Biological Chemistry·Juan Xicohtencatl-CortesJorge A Girón
Dec 15, 2012·Foodborne Pathogens and Disease·Aine M MonaghanDeclan J Bolton
Apr 12, 2003·The Pediatric Infectious Disease Journal·Joachim MisselwitzLudwig Patzer
Jul 6, 2000·Symposium Series·H Chart
Apr 23, 2013·Applied and Environmental Microbiology·Musafiri Karama, Carlton L Gyles
Jul 22, 2008·Applied and Environmental Microbiology·Mohammad A IslamAnnet E Heuvelink
Apr 27, 2002·Applied and Environmental Microbiology·Nathalie PradelValérie Livrelli
Mar 7, 2006·Applied and Environmental Microbiology·Lutz GeueFranz J Conraths
Jan 17, 2004·Clinical Microbiology Reviews·Herbert Schmidt, Michael Hensel

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.