PMID: 9192075Jan 1, 1997Paper

Essential arginine residues in isoprenylcysteine protein carboxyl methyltransferase

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
D BoivinR Béliveau

Abstract

We used specific amino acid modifying reagents to characterize the isoprenylcysteine carboxyl methyltransferase in kidney membranes. The enzyme was inactivated by reagents specific for arginine, histidine, cysteine, and tryptophan residues. Protection by the product and inhibitor S-adenosyl-L-homocysteine was observed for arginine modification by phenylglyoxal and tryptophan modification by N-bromosuccinimide. We focused on modification by phenylglyoxal, a highly specific modifier of arginine residues. The inactivation of methyltransferase by phenylglyoxal follows pseudo-first-order kinetics and the order of the reaction, n, with respect to phenylglyoxal was 1.2. The inactivation increased with the alkalinity of the preincubation medium and was maximal at pH 10. Kinetic analysis showed that the K(m) for S-adenosyl-L-methionine is not significantly affected by treatment with phenylglyoxal but that the Vmax is reduced p-Hydroxyphenylglyoxal, a more hydrophilic derivative of phenylglyoxal, was a less potent inactivator of methyltransferase than phenylglyoxal, suggesting that arginine residues modified are in a hydrophobic environment. The methyltransferase is protected from phenylglyoxal modification by S-adenosyl-L-homocysteine b...Continue Reading

References

Oct 31, 1975·Biochemistry·C L Borders, J F Riordan
Jan 1, 1992·Annual Review of Biochemistry·S Clarke
Jan 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·H K YamaneB K Fung
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D Pérez-SalaR R Rando
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H K YamaneJ A Glomset
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S ClarkeJ Stock
Sep 1, 1993·Pharmacology & Therapeutics·C A Hrycyna, S Clarke

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Citations

Nov 21, 2000·The International Journal of Biochemistry & Cell Biology·H M De BusserA R Lagrou
Apr 5, 2014·Biomechanics and Modeling in Mechanobiology·Jee E RimWilliam S Klug

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