Essential domain of receptor tyrosine phosphatase beta (RPTPbeta) for interaction with Helicobacter pylori vacuolating cytotoxin

The Journal of Biological Chemistry
Kinnosuke YahiroToshiya Hirayama

Abstract

Helicobacter pylori produces a potent exotoxin, VacA, which causes progressive vacuolation as well as gastric injury. Although VacA was able to interact with two receptor-like protein tyrosine phosphatases, RPTPbeta and RPTPalpha, RPTPbeta was found to be responsible for gastric damage caused by VacA. To define the region of RPTPbeta involved in VacA binding, we made mutants of human cDNA RPTPbeta-B, a short receptor form of RPTPbeta. Immunoprecipitation experiments to assess VacA binding to RPTPbeta-B mutants indicated that five residues (QTTQP) at positions 747-751 of the extracellular domain of RPTPbeta-B (which is commonly retained in RPTPbeta-A, a long form of RPTPbeta) play a crucial role in its interaction with VacA, resulting in vacuolation as well as Git-1 phosphorylation. Transfected cells expressing deletion mutant Delta752, which lacks QTTQP, or the double point mutant Delta747 (T748A,T749A) had diminished vacuolation in response to VacA. Treatment of RPTPbeta-B and Delta747 (which have QTTQP at 747-751) with neuraminidase and O-glycosidase diminished their VacA binding, whereas chondroitinase ABC did not have an effect. No inhibitory effect of pleiotrophin, a natural RPTPbeta ligand, on VacA binding to RPTPbeta-B o...Continue Reading

References

Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·N X Krueger, H Saito
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J SapJ Schlessinger
Jun 1, 1988·Journal of Medical Microbiology·R D LeunkD R Morgan
May 1, 1994·The Journal of Experimental Medicine·J L TelfordZ Xiang
Sep 23, 1997·Biochemical and Biophysical Research Communications·K YahiroT Hirayama
Jan 31, 1998·The Journal of Experimental Medicine·M MolinariC Montecucco
Jul 23, 1998·Infection and Immunity·P MassariJ L Telford
Nov 24, 1998·Infection and Immunity·M de BernardC Montecucco
Sep 26, 2000·Molecular and Cellular Biology·S HarrochJ Schlessinger
Feb 13, 2001·Biophysical Journal·T H Thanka Christlet, K Veluraja
Mar 13, 2001·Annual Review of Immunology·G Del GiudiceR Rappuoli
Oct 12, 2001·Toxicon : Official Journal of the International Society on Toxinology·E PapiniT L Cover
Mar 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Volaluck SupajaturaChisei Ra
Mar 21, 2002·Nature Reviews. Cancer·Richard M Peek, Martin J Blaser
Dec 31, 2002·Physiological Reviews·Karl G Johnson, David Van Vactor
Feb 4, 2003·The Journal of Biological Chemistry·Mark S McClainTimothy L Cover
Mar 1, 2003·Nature Genetics·Richard M Peek
Mar 11, 2003·The Journal of Biological Chemistry·Kinnosuke YahiroToshiya Hirayama
Nov 25, 2003·The Journal of Biological Chemistry·Masaaki NakayamaToshiya Hirayama
Dec 17, 2003·The Journal of Experimental Medicine·Marianna BoncristianoCosima T Baldari
May 7, 2004·Proceedings of the National Academy of Sciences of the United States of America·Mark S SundrudTimothy L Cover

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Citations

Jul 4, 2012·Journal of Proteome Research·Sandra PacharraIsabelle Breloy
Mar 11, 2005·Nature Reviews. Microbiology·Timothy L Cover, Steven R Blanke
Oct 13, 2005·Current Opinion in Gastroenterology·Hong LuDavid Y Graham
Nov 3, 2011·Cell Communication and Signaling : CCS·Joachim Rassow
Aug 30, 2013·Nihon saikingaku zasshi. Japanese journal of bacteriology·Masatoshi Noda
Jan 5, 2010·The Tohoku Journal of Experimental Medicine·Hajime IsomotoToshiya Hirayama
Sep 24, 2005·Helicobacter·Céu FigueiredoYoshio Yamaoka
Apr 24, 2016·Toxicon : Official Journal of the International Society on Toxinology·Muhammad JunaidGerd Katzenmeier
Jun 9, 2016·Toxins·Nora J FoegedingTimothy L Cover
Apr 15, 2020·Infection and Immunity·Rhonda R CastonTimothy L Cover
May 27, 2017·BioImpacts : BI·Farnaz FahimiYadollah Omidi
Apr 23, 2014·Chembiochem : a European Journal of Chemical Biology·Jin Yu, Ulrika Westerlind
Jan 12, 2008·Nihon saikingaku zasshi. Japanese journal of bacteriology·Toshiya Hirayama
Nov 21, 2020·International Journal of Molecular Sciences·Tariq FahmiAlexei G Basnakian
Feb 9, 2020·Seminars in Cell & Developmental Biology·Wei Yang SitWen-Ching Wang

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