Essential Dynamics of an Effective Phototherapeutic Drug in a Nanoscopic Delivery Vehicle: Psoralen in Ethosomes for Biofilm Treatment

ACS Omega
Damayanti BagchiSamir Kumar Pal

Abstract

Appropriate localization of a drug and its structure/functional integrity in a delivery agent essentially dictates the efficacy of the vehicle and the medicinal activity of the drug. In the case of a phototherapeutic drug, its photoinduced dynamics becomes an added parameter. Here, we have explored the photoinduced dynamical events of a model phototherapeutic drug psoralen (PSO) in a potential delivery vehicle called an ethosome. Dynamic light scattering confirms the structural integrity of the ethosome vehicle after the encapsulation of PSO. Steady state and picosecond resolved polarization gated spectroscopy, including the well-known strategy of solvation and Förster resonance energy transfer, reveal the localization of the drug in the vehicle and the environment in the proximity of PSO. We have also investigated the efficacy of drug delivery to various individual bacteria (Gram-negative: Escherichia coli; Gram-positive: Staphylococcus aureus) and bacterial biofilms. Our optical and electron microscopic studies reveal a significant reduction in bacterial survival (∼70%) and the destruction of bacterial adherence following a change in the morphology of the biofilms after phototherapy. Our studies are expected to find relevance...Continue Reading

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Citations

Jul 25, 2019·Current Pharmaceutical Design·Hiba NatshehElka Touitou
Jan 26, 2021·Royal Society Open Science·Saleh A AhmedSamir Kumar Pal
Mar 18, 2021·Drug Delivery and Translational Research·Tai WangXiaoliang Zhu
Feb 7, 2019·Bioconjugate Chemistry·Fangqin LiuShiyong Zhang
Oct 16, 2018·ACS Omega·Damayanti BagchiSamir Kumar Pal

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Methods Mentioned

BETA
dynamic light scattering
scanning electron microscopy
Fluorescence
FRET

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