Essential Function of the Serine Hydroxymethyl Transferase (SHMT) Gene During Rapid Syncytial Cell Cycles in Drosophila

G3 : Genes - Genomes - Genetics
Franziska WinklerJörg Großhans

Abstract

Many metabolic enzymes are evolutionarily highly conserved and serve a central function in the catabolism and anabolism of cells. The serine hydroxymethyl transferase (SHMT) catalyzing the conversion of serine and glycine and vice versa feeds into tetrahydrofolate (THF)-mediated C1 metabolism. We identified a Drosophila mutation in SHMT (CG3011) in a screen for blastoderm mutants. Embryos from SHMT mutant germline clones specifically arrest the cell cycle in interphase 13 at the time of the midblastula transition (MBT) and prior to cellularization. The phenotype is due to a loss of enzymatic activity as it cannot be rescued by an allele with a point mutation in the catalytic center but by an allele based on the SHMT coding sequence from Escherichia coli The onset of zygotic gene expression and degradation of maternal RNAs in SHMT mutant embryos are largely similar to that in wild-type embryos. The specific timing of the defects in SHMT mutants indicates that at least one of the SHMT-dependent metabolites becomes limiting in interphase 13, if it is not produced by the embryo. Our data suggest that mutant eggs contain maternally-provided and SHMT-dependent metabolites in amounts that suffice for early development until interphase...Continue Reading

References

Dec 26, 2001·Nucleic Acids Research·Ron EdgarAlex E Lash
Aug 16, 2003·Developmental Cell·Jörg GrosshansEric Wieschaus
Feb 7, 2006·Current Biology : CB·Annely BrandtJörg Grosshans
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Johannes BischofKonrad Basler
Jun 11, 2009·PloS One·Donald D Anderson, Patrick J Stover
Jan 12, 2010·Mechanisms of Development·Christian WenzlJörg Grosshans
Feb 25, 2011·PloS One·Livia VastagMarc W Kirschner
Aug 31, 2011·Proceedings of the National Academy of Sciences of the United States of America·Donald D AndersonPatrick J Stover
Oct 18, 2011·Integrative Biology : Quantitative Biosciences From Nano to Macro·Takuma KanesakiJörg Grosshans
Jan 12, 2012·The Journal of Biological Chemistry·Donald D AndersonPatrick J Stover
Jan 8, 2013·Current Biology : CB·Jeffrey A Farrell, Patrick H O'Farrell
Jan 8, 2013·Current Biology : CB·Stefano Di TaliaEric F Wieschaus
Dec 26, 2013·Developmental Biology·Sreemukta AcharyaJörg Großhans
Sep 10, 2014·Annual Review of Genetics·Jeffrey A Farrell, Patrick H O'Farrell
Sep 27, 2014·Genome Research·Nele A HaeltermanHugo J Bellen
Sep 4, 2015·Biophysical Journal·Franziska WinklerJörg Grosshans
Sep 12, 2015·Current Topics in Developmental Biology·Melissa M Harrison, Michael B Eisen
Sep 12, 2015·Current Topics in Developmental Biology·Shelby A Blythe, Eric F Wieschaus
May 1, 2017·Methods in Molecular Biology·Boyang Liu, Jörg Grosshans

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Citations

Jul 13, 2019·Archives of Insect Biochemistry and Physiology·Mohammad R HaqueKohji Yamamoto
Apr 10, 2019·Transplantation·Menghao WangJianping Gong
Sep 24, 2019·Cell Cycle·Boyang Liu, Jörg Großhans
Mar 20, 2021·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Lizzette D CambronKendra J Greenlee
Jul 3, 2021·Biology·Boyang LiuJörg Großhans

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Datasets Mentioned

BETA
GSE97557

Methods Mentioned

BETA
PCR
electrophoresis
gel filtration
light microscopy
RNAseq

Software Mentioned

NanoString
ImageJ
Photoshop
FIJI
NanoString nCounter

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