Essential gene profiles in breast, pancreatic, and ovarian cancer cells.

Cancer Discovery
Richard MarcotteJason Moffat

Abstract

Genomic analyses are yielding a host of new information on the multiple genetic abnormalities associated with specific types of cancer. A comprehensive description of cancer-associated genetic abnormalities can improve our ability to classify tumors into clinically relevant subgroups and, on occasion, identify mutant genes that drive the cancer phenotype ("drivers"). More often, though, the functional significance of cancer-associated mutations is difficult to discern. Genome-wide pooled short hairpin RNA (shRNA) screens enable global identification of the genes essential for cancer cell survival and proliferation, providing a "functional genomic" map of human cancer to complement genomic studies. Using a lentiviral shRNA library targeting ~16,000 genes and a newly developed, dynamic scoring approach, we identified essential gene profiles in 72 breast, pancreatic, and ovarian cancer cell lines. Integrating our results with current and future genomic data should facilitate the systematic identification of drivers, unanticipated synthetic lethal relationships, and functional vulnerabilities of these tumor types. This study presents a resource of genome-scale, pooled shRNA screens for 72 breast, pancreatic, and ovarian cancer cell...Continue Reading

References

Sep 13, 2001·Proceedings of the National Academy of Sciences of the United States of America·T SørlieA L Børresen-Dale
Dec 26, 2001·The American Journal of Pathology·O SaramäkiT Visakorpi
Dec 20, 2002·The New England Journal of Medicine·Marc J van de VijverRené Bernards
May 21, 2003·Breast Cancer Research and Treatment·Christopher J OrmandyRobert L Sutherland
Oct 28, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Hiroyuki OkamotoJohji Inazawa
Mar 5, 2004·British Journal of Cancer·K J SavinainenT Visakorpi
Mar 5, 2004·Cancer Cell·Anna D'AngeloMassimo Zollo
Apr 13, 2004·Proceedings of the National Academy of Sciences of the United States of America·Andrew I SuJohn B Hogenesch
Jul 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Adam AmsterdamNancy Hopkins
Dec 21, 2004·Nucleic Acids Research·Kevin P O'BrienErik L L Sonnhammer
Feb 8, 2005·The International Journal of Biochemistry & Cell Biology·Malte Bachmann, Tarik Möröy
Oct 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Aravind SubramanianJill P Mesirov
Nov 22, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ali O GureNasser K Altorki
Jan 24, 2006·Molecular and Cellular Biology·Tsuyoshi KobayashiAkira Kikuchi
Feb 24, 2006·Nature Reviews. Molecular Cell Biology·Jason Moffat, David M Sabatini
Apr 22, 2006·Cellular Signalling·Wolfgang F VogelCaroline E Ford
Mar 14, 2007·Trends in Pharmacological Sciences·Jason MoffatDavid M Sabatini
May 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Kwang-Il GohAlbert-László Barabási
Jul 25, 2007·Molecular and Cellular Biology·Hongyan WangChristopher E Rudd
Aug 3, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Sunil BadveHarikrishna Nakshatri
Sep 11, 2007·Nature Methods·Renate KönigSumit K Chanda
Oct 13, 2007·Science·Laura D WoodBert Vogelstein
Feb 2, 2008·Science·Michael R SchlabachStephen J Elledge
Jul 2, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Alan Ashworth
Dec 19, 2008·Proceedings of the National Academy of Sciences of the United States of America·Biao LuoDavid E Root
Feb 11, 2009·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Federico CappuzzoRobert Gemmill
Aug 20, 2009·Cell Cycle·Anurag Singh, Jeff Settleman
Dec 18, 2009·Nature·Erin D PleasanceMichael R Stratton

❮ Previous
Next ❯

Citations

Sep 4, 2012·Immunologic Research·Francesca Alvarez-CalderonJames DeGregori
Jul 16, 2013·Nature Communications·Xiaowen LuRichard A Notebaart
May 1, 2013·Nature Methods·Traver Hart, Jason Moffat
Jul 17, 2013·The Journal of Experimental Medicine·Chiara GorriniMona L Gauthier
Jul 3, 2013·Annual Review of Genomics and Human Genetics·Anastasia BaryshnikovaCharles Boone
Sep 18, 2013·BMC Genomics·A Hunter ShainJonathan R Pollack
Feb 13, 2013·BMC Medical Genomics·Fan Zhang, Jake Y Chen
May 16, 2013·Genome Medicine·Alvin Makohon-MooreChristine A Iacobuzio-Donahue
Dec 3, 2014·Trends in Molecular Medicine·James M PaulFranco J Vizeacoumar
Oct 18, 2014·Nature Reviews. Drug Discovery·Adrienne D CoxChanning J Der
Dec 3, 2015·Critical Reviews in Biochemistry and Molecular Biology·Tianzuo Zhan, Michael Boutros
Dec 15, 2015·Clinical Pharmacology and Therapeutics·B Chen, A J Butte
Jan 30, 2016·Nature Reviews. Cancer·Emmy D G FleurenRoger J Daly
Sep 16, 2015·Proceedings of the National Academy of Sciences of the United States of America·Wout MegchelenbrinkRichard A Notebaart
Feb 24, 2016·Nature Chemical Biology·Satra NimPhilip M Kim
Nov 28, 2015·Science·Charles Boone, Brenda J Andrews
Oct 1, 2013·Protein & Cell·Meng-Tzu Weng, Ji Luo
Dec 1, 2015·Nature Genetics·Mauro A A CastroKerstin B Meyer
Dec 17, 2015·Pharmacogenomics·Aik Choon TanPaul H Huang
Mar 8, 2016·Cell Reports·James CampbellChristopher J Lord
Dec 9, 2015·Breast Cancer Research : BCR·Preeti PutchaJose Silva
Oct 10, 2013·Molecular Systems Biology·Franco J VizeacoumarJason Moffat
Mar 26, 2016·Surgical Oncology Clinics of North America·Vincent BernardAnirban Maitra
Sep 4, 2015·Nature·Tiffany Y-T HsuThomas F Westbrook
Jun 10, 2015·Integrative Biology : Quantitative Biosciences From Nano to Macro·Mark F CiaccioNeda Bagheri
Feb 15, 2014·Cancer Cell·Jane CullisRobert Rottapel

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Genomics (Keystone)

Cancer genomics approaches employ high-throughput technologies to identify the complete catalog of somatic alterations that characterize the genome, transcriptome and epigenome of cohorts of tumor samples. Discover the latest research using such technologies in this feed.

Birth Defects

Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.