Essential genes of the macrophage response to Staphylococcus aureus exposure

Cellular & Molecular Biology Letters
Aixia SunJian Zhang

Abstract

Although significant advances have been made in understanding the mechanisms of macrophage response to Staphylococcus aureus infection, the molecular details are still elusive. Identification of the essential genes and biological processes of macrophages that are specifically changed at different durations of S. aureus exposure is of great clinical significance. We aimed to identify the significantly changed genes and biological processes of S. aureus-exposed macrophages. We systematically analyzed the macrophage gene expression profile GSE 13670 database with 8 h, 24 h or 48 h S. aureus infection. The results were further confirmed by western blot and quantitative polymerase chain reaction (qPCR) analyses. After 8 h of S. aureus infection, the expression of 624 genes was significantly changed. Six hundred thirteen differentially expressed genes (DEGs) were identified after 24 h of S. aureus infection. Two hundred fifty-three genes were significantly changed after 48 h of S. aureus infection. STAT1 was consistently up-regulated in these three treatments. TP53, JAK2, CEBPA, STAT3, MYC, CTNNB1 and PRKCA were only identified in the 8 h or 24 h S. aureus infection groups. CTNNB1 and PRKCA were for the first time identified as poten...Continue Reading

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Citations

Feb 6, 2021·Frontiers in Immunology·Grace R PidwillSimon J Foster

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Datasets Mentioned

BETA
GSE
13670

Methods Mentioned

BETA
transfection
PCR

Software Mentioned

gplots
Limma
ClusterProfiler
Cytoscape
R
genefilter
GCRMA

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