Abstract
Novel subfamily members of deubiquitinating enzyme gene, DUB-1 and DUB-2, are known to be immediate-early genes and are specifically expressed in B-lymphocytes and T-lymphocytes, respectively. Recently, another deubiquitinating enzyme gene DUB-2A, expressed also in T-lymphocytes, has been isolated and we, in this study, found that Dub-2A has isopeptidase activity and there are at least 4 conserved amino acids (Cys60, Asp133, His298, and His307) that are required for catalytic activity of the enzyme. Interestingly, the conserved Asp323 was not essential for the catalytic activity. In addition, transcriptional reporter assay showed that an Ets site and 2 AP-1 sites in the enhancer domain of this gene are required for cytokine inducibility. Taken all together, these results will be very helpful in designing activators or inhibitors of this enzyme in order to regulate its expression and catalytic activity in T-lymphocytes.
References
Jan 1, 1992·Annual Review of Biochemistry·A Hershko, A Ciechanover
Jan 1, 1992·Annual Review of Genetics·S Jentsch
Aug 1, 1995·Current Biology : CB·D M Rubin, D Finley
Jan 5, 1995·Nature·M ScheffnerJ M Huibregtse
Nov 25, 1993·Nature·F R Papa, M Hochstrasser
Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·Y ZhuA D D'Andrea
Jan 1, 1996·Annual Review of Biochemistry·O CouxA L Goldberg
Jan 3, 1997·The Journal of Biological Chemistry·Y ZhuA D D'Andrea
Jun 1, 1997·Molecular and Cellular Biology·R JasterA D D'Andrea
Nov 25, 1998·Critical Reviews in Biochemistry and Molecular Biology·A D'Andrea, D Pellman
Dec 19, 1998·The EMBO Journal·A Ciechanover
Oct 19, 1999·Biochimica Et Biophysica Acta·R JasterA D D'Andrea
Dec 22, 1999·Biochemical and Biophysical Research Communications·C H Chung, S H Baek
Jul 8, 2000·Trends in Cell Biology·S Jentsch, G Pyrowolakis
Jul 25, 2000·Seminars in Cell & Developmental Biology·K D Wilkinson
Jul 10, 2001·American Journal of Hematology·J H LeeK H Baek
Jul 27, 2001·Blood·K H BaekA D D'Andrea
Nov 26, 2002·American Journal of Hematology·Kwang-Hyun Baek
Jan 1, 2003·Cell·Min HuYigong Shi
Mar 19, 2003·Experimental & Molecular Medicine·Kwang-Hyun Baek
Apr 19, 2003·The Journal of Biological Chemistry·Paul C EvansPeter J Kilshaw
Apr 22, 2003·EMBO Reports·Maxim Y BalakirevJadwiga Chroboczek
May 22, 2003·The Journal of Biological Chemistry·Tudeviin Gan-ErdeneKeith D Wilkinson
Oct 30, 2003·The Journal of Biological Chemistry·Kwang-Hyun BaekHee-Kyung Choi
Apr 8, 2004·Molecular Cell·Xiao-Ming SunGerald M Cohen
Citations
Aug 19, 2010·Hybridoma·Suresh RamakrishnaKwang-Hyun Baek
Apr 6, 2005·Biochemical and Biophysical Research Communications·Myung-Sun KimKwang-Hyun Baek
Dec 16, 2010·The Biochemical Journal·Annemarie MeenhuisIvo P Touw
Apr 19, 2019·Journal of Applied Physiology·Shane M HammerThomas J Barstow