Essential role for cathepsin S in MHC class II-associated invariant chain processing and peptide loading

Immunity
R J RieseH A Chapman

Abstract

Destruction of li by proteolysis is required for MHC class II molecules to bind antigenic peptides, and for transport of the resulting complexes to the cell surface. The cysteine protease cathepsin S is highly expressed in spleen, lymphocytes, monocytes, and other class II-positive cells, and is inducible with interferon-gamma. Specific inhibition of cathepsin S in B lymphoblastoid cells prevented complete proteolysis of li, resulting in accumulation of a class II-associated 13 kDa li fragment in vivo. Consequently, the formation of SDS-stable complexes was markedly reduced. Purified cathepsin S, but not cathepsin B, H, or D, specifically digested li from alpha beta li trimers, generating alpha beta-CLIP complexes capable of binding exogenously added peptide in vitro. Thus, cathepsin S is essential in B cells for effective li proteolysis necessary to render class II molecules competent for binding peptides.

References

Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·P A Roche, P Cresswell
Mar 1, 1989·Human Immunology·Q V NguyenR E Humphreys
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J S Blum, P Cresswell
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J I BellH O McDevitt
Feb 15, 1985·The Biochemical Journal·R W MasonA J Barrett
Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·L ClaessonP A Peterson
Jan 1, 1984·The Biochemical Journal·R W MasonD J Etherington
Jan 1, 1981·Methods in Enzymology·A J Barrett, H Kirschke
Aug 1, 1994·The Journal of Experimental Medicine·M J BijlmakersH L Ploegh
Jan 3, 1995·The EMBO Journal·P BénarochH L Ploegh
Jun 29, 1995·Nature·V S SloanD M Zaller
Aug 18, 1995·Journal of Medicinal Chemistry·J T PalmerD Brömme
Apr 1, 1995·Journal of Leukocyte Biology·W P LafuseB S Zwilling
Mar 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·M A MarićJ S Blum
Jan 1, 1995·Annual Review of Cell and Developmental Biology·P R Wolf, H L Ploegh
Apr 1, 1996·Protein Science : a Publication of the Protein Society·D Brömme, M E McGrath

❮ Previous
Next ❯

Citations

Oct 3, 1999·European Journal of Immunology·P W BryantA J Sant
Mar 10, 2001·International Journal of Cancer. Journal International Du Cancer·P L FernándezA Cardesa
Jul 9, 1998·Protein Science : a Publication of the Protein Society·M E McGrathJ R Somoza
Jan 1, 1997·Immunologic Research·S O ArndtH Kropshofer
Mar 18, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·Izabela Berdowska
Sep 19, 2003·International Journal for Parasitology·S Hartmann, R Lucius
Jun 1, 1997·Immunology Today·H L Ploegh
Feb 11, 1999·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·M Synnes
Jan 13, 2001·Current Opinion in Immunology·C Watts
Jan 13, 2001·Current Opinion in Immunology·C Théry, S Amigorena
Jan 16, 2002·Current Opinion in Immunology·Ana-Maria Lennon-DuménilCécile Lagaudrière-Gesbert
Feb 1, 1997·Current Opinion in Immunology·J Pieters
Apr 2, 1998·Current Opinion in Immunology·H A Chapman
May 29, 1998·Current Opinion in Immunology·P Pierre, I Mellman
Feb 19, 2000·Current Opinion in Immunology·R J Riese, H A Chapman
Mar 1, 1997·Trends in Cell Biology·R WubboltsJ Neefjes
Jan 23, 2003·Bioorganic & Medicinal Chemistry·Charles L CywinDenice M Spero
Mar 26, 2003·Protein Expression and Purification·Sherry M BakerRobin L Thurmond
Mar 15, 2000·The International Journal of Biochemistry & Cell Biology·R I BrinkworthJ P Dalton
Nov 26, 2008·The Journal of Biological Chemistry·Susan R WilsonDieter Brömme
Oct 30, 2008·The Journal of Biological Chemistry·Nicolas Lützner, Hubert Kalbacher
Aug 21, 2002·The Journal of Experimental Medicine·Ana-Maria Lennon-DuménilCécile Lagaudrière-Gesbert
Jan 23, 2003·The Journal of Experimental Medicine·Courtney BeersAlexander Rudensky
May 27, 2009·The Journal of Experimental Medicine·Ildiko Van RhijnD Branch Moody
Dec 26, 2012·The Journal of Experimental Medicine·Daniel R BeisnerMichael P Cooke

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.