Essential role for gene profiling analysis in the authentication of human cell lines.

Human Cell
Kaori YoshinoYukio Nakamura

Abstract

Cross-contamination between cultured cell lines can result in the generation of erroneous scientific data. Hence, it is very important to eliminate cell lines that are of an origin different from that being claimed. Inter-species contamination can be detected by various established methods, such as karyotype and isozyme analyses. However, it has been impossible to detect intraspecies cross-contamination prior to the development of technology to detect differences between cell lines at the molecular level. Recently, profiling of short tandem repeat (STR) polymorphisms has been established as a method for the analyses of gene polymorphism. Gene profiling by STR polymorphism (STR profiling) is a simple and reliable method to identify individual cell lines. Each human cell line currently provided by the Cell Engineering Division of the RIKEN BioResource Center was analyzed by STR profiling to authenticate its identity. We found that more than 10 human cell lines out of approximately 400 were in fact identical to a different cell line deposited in the collection, and therefore had been misidentified. We conclude that STR profiling is a useful and powerful method for eliminating cell lines that have been misidentified by cross-contam...Continue Reading

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Citations

Jun 17, 2008·In Vitro Cellular & Developmental Biology. Animal·Merry LiuAbbas Vafai
Jul 10, 2013·Cancer Letters·Yi-Ting ChenChuan-Fa Chang
Jun 21, 2008·Acta Pharmacologica Sinica·Armando RojasHéctor Figueroa
Jul 25, 2009·Human Cell·Inaho DanjohYukio Nakamura
Oct 27, 2010·Human Cell·Tsuyoshi FujiokaYukio Nakamura
Dec 7, 2013·Nucleic Acids Research·Harald StachelscheidAndreas Kurtz
May 11, 2010·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Albiruni R A RazakKelvin Chan
Nov 19, 2008·International Immunopharmacology·Armando RojasHector Figueroa
Oct 26, 2007·International Journal of Cancer. Journal International Du Cancer·Marc Lacroix
Feb 10, 2010·International Journal of Cancer. Journal International Du Cancer·Amanda Capes-DavisR Ian Freshney
Mar 21, 2015·The American Journal of Pathology·Zaki ShaikhibrahimSven Perner
May 27, 2010·British Journal of Cancer·P WorkmanUNKNOWN Committee of the National Cancer Research Institute
Mar 13, 2010·Experimental Animals·Yukio Nakamura

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