Essential role of endophilin A in synaptic vesicle budding at the Drosophila neuromuscular junction

The EMBO Journal
Antoine GuichetAnne A Schmidt

Abstract

We characterized Drosophila endophilin A (D-endoA), and generated and analysed D-endoA mutants. Like its mammalian homologue, D-endoA exhibits lysophosphatidic acid acyl transferase activity and contains a functional SH3 domain. D-endoA is recruited to the sites of endocytosis, as revealed by immunocytochemistry of the neuromuscular junction (NMJ) of mutant L3 larvae carrying the temperature-sensitive allele of dynamin, shibire. D-endoA null mutants show severe defects in motility and die at the early L2 larval stage. Mutants with reduced D-endoA levels exhibit a range of defects of synaptic vesicle endocytosis, as observed at L3 larvae NMJs using FM1-43 uptake and electron microscopy. NMJs with an almost complete loss of synaptic vesicles did not show an accumulation of intermediates of the budding process, whereas NMJs with only slightly reduced levels of synaptic vesicles showed a striking increase in early-stage, but not late-stage, budding intermediates at the plasma membrane. Together with results of previous studies, these observations indicate that endophilin A is essential for synaptic vesicle endocytosis, being required from the onset of budding until fission.

References

Mar 21, 1997·Cell·M González-Gaitán, H Jäckle
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·N RingstadP De Camilli
Oct 7, 1997·FEBS Letters·K D MichevaP S McPherson
Oct 29, 1998·Methods : a Companion to Methods in Enzymology·A Schmidt, W B Huttner
Dec 28, 1999·Annual Review of Cell and Developmental Biology·M J HannahW B Huttner
Aug 31, 2000·Annual Review of Biochemistry·T Kirchhausen
Mar 22, 2001·Nature Reviews. Neuroscience·V I Slepnev, P De Camilli
Aug 8, 2001·Trends in Cell Biology·F ValtortaR Fesce
Oct 18, 2001·The Journal of Cell Biology·K FarsadP De Camilli
Oct 18, 2001·The Journal of Cell Biology·X WuL E Greene
Apr 30, 2002·Trends in Cell Biology·Wieland B Huttner, Anne A Schmidt

❮ Previous
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Citations

Jun 21, 2006·Cell and Tissue Research·Lennart Brodin, Oleg Shupliakov
Dec 25, 2010·Cell Biochemistry and Biophysics·Ole KjaerulffAnita Jung
Mar 17, 2004·Journal of Neuroscience Methods·Emma EvergrenOleg Shupliakov
Oct 15, 2003·Trends in Molecular Medicine·Jia-Yi LiPatrik Brundin
Aug 2, 2003·Current Opinion in Cell Biology·Khashayar Farsad, Pietro De Camilli
Oct 9, 2002·Current Opinion in Neurobiology·Janet E Richmond, Kendal S Broadie
Apr 30, 2002·Trends in Cell Biology·Wieland B Huttner, Anne A Schmidt
Feb 3, 2005·Nature Reviews. Neuroscience·Jeffrey Rohrbough, Kendal Broadie
Jan 8, 2010·The Journal of Biological Chemistry·Anna GrygorukDavid E Krantz
Dec 23, 2004·Science's STKE : Signal Transduction Knowledge Environment·Thierry Galli, Volker Haucke
Mar 10, 2006·Microbiology and Molecular Biology Reviews : MMBR·Gang RenAlan L Munn
Oct 7, 2003·Annual Review of Neuroscience·Venkatesh N Murthy, Pietro De Camilli
Sep 10, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jeffrey RohrboughKendal Broadie
Jun 10, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Artur LlobetHarvey T McMahon
Oct 17, 2002·Proceedings of the National Academy of Sciences of the United States of America·Oleg ShupliakovLennart Brodin
Jan 1, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Mark ClementsJeffrey Milbrandt
Sep 3, 2010·Journal of Neurogenetics·Riddhi Majumder, K S Krishnan
Jun 28, 2005·FEBS Letters·Yuko AramakiSoichi Takiguchi
Nov 11, 2003·Journal of Neurobiology·Maryse NicolaïJean-Maurice Dura
Sep 10, 2003·The Journal of Physiology·Stephen J Royle, Leon Lagnado
Sep 17, 2005·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Takako KanekoKozo Kaibuchi
May 10, 2007·Traffic·Evan Eisenberg, Lois E Greene
May 30, 2008·Traffic·Hao FeiDavid E Krantz
Jun 10, 2006·The EMBO Journal·Jennifer L GallopHarvey T McMahon
Jul 25, 2007·Traffic·Silvio O Rizzoli, Reinhard Jahn
Jun 2, 2011·Developmental Neurobiology·Eugene Oh, Iain Robinson
May 13, 2011·Developmental Neurobiology·Giovanni EspositoPatrik Verstreken
Jun 30, 2006·Current Opinion in Cell Biology·Tom J Brett, Linton M Traub
Dec 19, 2015·Neural Plasticity·Jifeng ZhangYuan Chen
May 20, 2006·Experimental Cell Research·Amitabha MajumdarRicha Rikhy
Aug 2, 2005·Brain Research. Brain Research Reviews·María Luz MontesinosRafael Fernández-Chacón
Jul 19, 2005·Journal of Molecular Biology·Winfried Weissenhorn
Sep 25, 2012·Neuron·Samer MattaPatrik Verstreken
Nov 19, 2003·Neuron·Kimberly R SchuskeErik M Jorgensen
Nov 19, 2003·Neuron·Patrik VerstrekenHugo J Bellen

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