PMID: 9539747May 16, 1998Paper

Essential role of germinal vesicle material in the meiotic cell cycle of Xenopus oocytes

Proceedings of the National Academy of Sciences of the United States of America
J IwashitaN Sagata

Abstract

In almost all animal species, immature oocytes are arrested naturally in the first meiotic prophase, with a large nucleus called the germinal vesicle. A number of previous studies showed that both activation of maturation/M phase-promoting factor (MPF) (assayed by semiquantitative cytological methods) and some other maturational events occur essentially normally in enucleated oocytes from many amphibian species and mice. Hence, for nearly three decades, it has generally been believed that nuclear material is dispensable for MPF activation and the meiotic cell cycle in vertebrate oocytes. Here, we have challenged this view by examining the histone H1 kinase activities and the molecular forms of MPF in experimentally manipulated Xenopus oocytes. We show that oocytes injected with nuclear material undergo much more rapid MPF activation and maturation than uninjected control oocytes. Conversely, enucleated oocytes, unlike nucleated counterparts, undergo only weak MPF activation in meiosis I and no detectable MPF reactivation in meiosis II, the latter accompanying inhibitory tyrosine phosphorylation of cdc2 kinase, the catalytic subunit of MPF. These results argue strongly that nuclear material is indispensable for the meiotic cell ...Continue Reading

References

Jul 1, 1979·Journal of Steroid Biochemistry·Y MasuiD H Ziegler
Jan 1, 1979·International Review of Cytology·Y Masui, H J Clarke
Dec 1, 1977·Experimental Cell Research·H Balakier, R Czolowska
May 1, 1976·Developmental Biology·C Katagiri, M Moriya
Jan 1, 1992·Molecular Biology of the Cell·M J SolomonM W Kirschner
Jan 1, 1989·Free Radical Biology & Medicine·F UrsiniL Ernster
Dec 1, 1988·Experimental Cell Research·C M FeldherrP Hodges
Jun 1, 1971·The Journal of Experimental Zoology·Y Masui, C L Markert
Mar 1, 1969·Developmental Biology·L D Smith, R E Ecker
Jun 1, 1984·Cell Differentiation·M N SkoblinaO T Kondratieva
Jul 1, 1980·Experimental Cell Research·H Bałakier, A K Tarkowski
Mar 29, 1993·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R A LaskeyC Dingwall
Jan 1, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·N Sagata
Jul 1, 1997·The EMBO Journal·N FurunoN Sagata

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Citations

Sep 25, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J E Ferrell
Jan 9, 2003·Current Opinion in Cell Biology·Péter Lénárt, Jan Ellenberg
Sep 13, 2012·Nature Communications·Masatoshi HaraTakeo Kishimoto
Jun 21, 2007·Cloning and Stem Cells·Junya ItoMasumi Hirabayashi
Mar 13, 2003·Molecular Biology of the Cell·Clare BeckhellingEvelyn Houliston
Jul 22, 1998·The EMBO Journal·A HagtingJ Pines
Aug 24, 2002·Biology of Reproduction·Shaorong GaoIan Wilmut
Dec 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·J TianJ V Ruderman
Jan 26, 2013·Development, Growth & Differentiation·Jun IwashitaJun Murata
Sep 24, 1999·Developmental Biology·T Kishimoto
Oct 26, 2013·Advances in Biological Regulation·Kenza Snoussi, Michael Kann
May 17, 2005·Developmental Biology·Zbigniew PolanskiChizuko Tsurumi
Apr 7, 2010·Current Biology : CB·E Josué RuizAngel R Nebreda
Jan 9, 2001·Nature Cell Biology·J Pines, C L Rieder

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