Essential role of Hand2 in interventricular septum formation and trabeculation during cardiac development

Biochemical and Biophysical Research Communications
Kiyonori TogiMakoto Tanaka

Abstract

Interventricular septum (IVS) formation is one of the key events in the development of a four-chambered heart. We previously showed that the basic helix-loop-helix transcription factor Hand1 plays an important role in the formation of the IVS. Here, we found that the other Hand gene, Hand2, regulated expansion, trabeculation, and IVS formation in the embryonic heart. In transgenic embryos expressing Hand2 in the whole ventricles, the boundary region between the left and right ventricles expanded outwards, resulting in complete absence of the IVS. Moreover, trabecular formation was observed even in a region where the IVS was expected to form. In some transgenic embryos with heterogeneous expression of the transgene, a muscular septum did not form in a region where Hand2 was expressed, but an incomplete septum was identifiable in a region where Hand2 was not expressed, suggesting that septum formation was strictly regulated by the expression domain of Hand2. Furthermore, expression of trabecular markers including ANF, BNP, and connexin40 was significantly up-regulated in the ventricles of Hand2 transgenic embryos as well as in H9c2 cells over-expressing Hand2. These results suggested that the absence of Hand2 expression in the in...Continue Reading

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Citations

Apr 21, 2009·The International Journal of Developmental Biology·Ralston M Barnes, Anthony B Firulli
Feb 24, 2007·Biochemical and Biophysical Research Communications·Yoshinori YoshidaMakoto Tanaka
Jul 30, 2016·Frontiers in Bioengineering and Biotechnology·Romina D'AurizioMarco Pellegrini
Apr 5, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Hironobu MatsumaeMakoto Tanaka
Oct 6, 2018·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Rajani M George, Anthony B Firulli
Aug 19, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Rumi MaruyamaHisayoshi Fujiwara
Mar 12, 2021·Current Cardiology Reports·Matthew MiyamotoEmmanouil Tampakakis
Jun 2, 2021·Annual Review of Genomics and Human Genetics·Lucile Houyel, Sigolène M Meilhac

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