Abstract
Mycobacterium leprae (M. leprae), the causative agent of leprosy, parasitizes within the foamy or enlarged phagosome of macrophages where rich lipids accumulate. Although the mechanisms for lipid accumulation in the phagosome have been clarified, it is still unclear how such large amounts of lipids escape degradation. To further explore underlying mechanisms involved in lipid catabolism in M. leprae-infected host cells, we examined the expression of hormone-sensitive lipase (HSL), a key enzyme in fatty acid mobilization and lipolysis, in human macrophage THP-1 cells. We found that infection by live M. leprae significantly suppressed HSL expression levels. This suppression was not observed with dead M. leprae or latex beads. Macrophage activation by peptidoglycan (PGN), the ligand for toll-like receptor 2 (TLR2), increased HSL expression; however, live M. leprae suppressed this increase. HSL expression was abolished in the slit-skin smear specimens from patients with lepromatous and borderline leprosy. In addition, the recovery of HSL expression was observed in patients who experienced a lepra reaction, which is a cell-mediated, delayed-type hypersensitivity immune response, or in patients who were successfully treated with mult...Continue Reading
References
Jun 1, 1976·Japanese Journal of Medical Science & Biology·E Kondo, K Kanai
Jul 20, 1990·European Journal of Biochemistry·A J Garton, S J Yeaman
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J ChanB R Bloom
Feb 7, 1998·The Journal of Biological Chemistry·M W AnthonsenC Holm
May 25, 1999·Cell·G FerrariJ Pieters
Aug 10, 2000·Scandinavian Journal of Immunology·P J CardonaV Ausina
Nov 21, 2000·The Journal of Biological Chemistry·N E WolinsD L Brasaemle
Mar 10, 2001·Nature·S T ColeB G Barrell
Jan 17, 2002·Microbiology and Immunology·S SasakiN Ishii
May 1, 2002·Nihon Hansenbyō Gakkai zasshi = Japanese journal of leprosy : official organ of the Japanese Leprosy Association·Yasuko YogiHiroko Nomaguchi
Jun 22, 2002·The Journal of Biological Chemistry·Shinji MiuraAlan R Kimmel
Apr 15, 2003·Nature Medicine·Stephan R KrutzikRobert L Modlin
Jun 18, 2003·The Journal of Cell Biology·Carole SztalrydConstantine Londos
Jul 2, 2003·The Journal of Biological Chemistry·Chun-Li SuConstantine Londos
Apr 3, 2004·Journal of Lipid Research·Mohamad NavabAlan M Fogelman
May 25, 2004·Antimicrobial Agents and Chemotherapy·Keisuke SanoToshiaki Shimizu
Aug 5, 2004·The Journal of Biological Chemistry·Vidya SubramanianDawn L Brasaemle
Mar 1, 2007·Acta Histochemica Et Cytochemica·Koichi SuzukiMasahiko Makino
Mar 16, 2007·The Journal of Clinical Endocrinology and Metabolism·Johan W E JockenEllen E Blaak
Mar 21, 2007·Tuberculosis·Robert L HunterJeffrey K Actor
Jul 3, 2007·Cell·Nicole van der WelPeter J Peters
Jul 19, 2008·The Journal of Clinical Investigation·Daniel CruzRobert L Modlin
Nov 13, 2008·PLoS Pathogens·Pascale PeyronFrédéric Altare
Nov 18, 2008·Biochimica Et Biophysica Acta·Robert ZimmermannRudolf Zechner
Dec 5, 2008·FEMS Microbiology Letters·Kazunari TanigawaNorihisa Ishii
Mar 17, 2009·Journal of Bacteriology·Takeshi AkamaNorihisa Ishii
May 15, 2009·Clinical and Experimental Immunology·K TanigawaN Ishii
Sep 1, 2009·Journal of Bacteriology·Anna BrzostekJaroslaw Dziadek
Jun 18, 2010·Microbial Pathogenesis·Takeshi AkamaKoichi Suzuki
Jul 3, 2010·EMBO Molecular Medicine·Mi-Jeong KimDavid G Russell
Aug 17, 2010·Biochemical and Biophysical Research Communications·Junetsu OgasawaraHideki Ohno
Citations
Dec 14, 2012·PLoS Neglected Tropical Diseases·Yang DegangKoichi Suzuki
Dec 18, 2013·Future Microbiology·Yang DegangKoichi Suzuki
Dec 21, 2014·BMC Immunology·Yuqian LuoKoichi Suzuki
Dec 5, 2015·Journal of Mass Spectrometry : JMS·Cristiana S de MacedoMaria Cristina V Pessolani
Feb 21, 2014·Cellular Microbiology·Katherine A MattosMaria Cristina V Pessolani
Mar 17, 2015·Cellular Microbiology·Caroline BarischThierry Soldati
Mar 14, 2017·Future Microbiology·Gurkamaljit Kaur, Jagdeep Kaur
Oct 5, 2017·The British Journal of Dermatology·D WangY-G Yao
Jan 31, 2014·Infection and Immunity·Olivier Neyrolles
Jul 23, 2014·Antimicrobial Agents and Chemotherapy·Lívia Silva LobatoFlávio Alves Lara
Aug 29, 2013·Current Opinion in Infectious Diseases·Anastasia PolycarpouDiana N Lockwood
Sep 26, 2019·Journal of Medical Microbiology·Ruchi RastogiJagdeep Kaur
Oct 20, 2020·PLoS Neglected Tropical Diseases·Yuqian LuoKoichi Suzuki
Mar 27, 2021·PloS One·Kazunari TanigawaKoichi Suzuki
Jul 25, 2021·International Journal of Molecular Sciences·Kazunari TanigawaKoichi Suzuki