Essential role of pepsin in pathogenesis of acid reflux esophagitis in rats

Digestive Diseases and Sciences
Kenji NagahamaKoji Takeuchi

Abstract

Pepsin, a protease activated by gastric acid, is a component of the refluxate, yet the role of pepsin in the pathogenesis of reflux esophagitis has not been well studied. In the present study, we examined the effect of pepstatin, a specific inhibitor of pepsin, on acid reflux esophagitis. Acid reflux esophagitis was induced in rats by ligating both the pylorus and the forestomach for 3 or 4 hr. Pepstatin, ecabet Na (the anti-ulcer drug), and L-glutamine were administered intragastrically after the ligation. Pepstatin or ecabet Na, given intragastrically, significantly prevented esophageal lesions, even though they did not affect basal acid secretion in pylorus-ligated rats. Pepstatin significantly inhibited pepsin activity in vivo and in vitro, while ecabet Na inhibited this activity in vitro. By contrast, L-glutamine given intragastrically aggravated the lesions in a dose-dependent manner, but even in the presence of L-glutamine the development of esophageal lesions was totally prevented by coadministration of pepstatin or ecabet Na. L-Glutamine increased the pH of gastric contents to approximately 2.0, the optimal pH for the proteolytic activity of pepsin in vitro. In addition, intragastric administration of exogenous pepsin ...Continue Reading

References

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Citations

Apr 10, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Chao QinRobert D Foreman
Dec 31, 2011·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Kenji NagahamaKoji Takeuchi
Jul 25, 2009·World Journal of Gastroenterology : WJG·Daisuke AsaokaSumio Watanabe
Mar 4, 2014·World Journal of Gastroenterology : WJG·Zullyt ZamoraAmbar Oyarzabal
Dec 4, 2010·Best Practice & Research. Clinical Gastroenterology·Philip Woodland, Daniel Sifrim
Mar 31, 2007·Life Sciences·Tohru KotaniKoji Takeuchi
Aug 31, 2006·Alimentary Pharmacology & Therapeutics·N B Roberts
Feb 24, 2015·Translational Research : the Journal of Laboratory and Clinical Medicine·Harit KapoorSumeet K Mittal
Mar 29, 2014·BioMed Research International·Koji Takeuchi, Kenji Nagahama
Feb 13, 2009·European Journal of Gastroenterology & Hepatology·Thomas WexPeter Malfertheiner
Jun 4, 2019·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Boyun QiuXiaohua Hou
Sep 26, 2019·Scientific Reports·Bryan P HurleyRachel Rosen
Nov 4, 2009·Scandinavian Journal of Gastroenterology·Kazuto TsuboiKatsuhiko Yanaga
Oct 24, 2020·International Journal of Biological Macromolecules·Sajad MoradiMohsen Shahlaei

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