Essential role of the beta subunit in modulation of C-class L-type Ca2+ channels by intracellular pH

FEBS Letters
K SchuhmannK Groschner

Abstract

Elevation of intracellular pH (pHi) enhances the activity of native L-type Ca2+ channels in cardiac and smooth muscle. We studied the modulation by pHi of expressed L-type Ca2+ channels comprised of either the alpha1c subunits alone or of alpha1c plus beta2a subunits. Ca2+ channels were expressed in human embryonic kidney cells (HEK 293) and pHi was increased from a basal level of 7.3 to 8.3 by exposure of cells to NH4Cl (20 mM) or by elevation of extracellular pH to 8.5. Elevation of pHi enhanced the activity of Ca2+ channels derived by coexpression of alpah1c and beta2a subunits. This alkalosis-induced stimulation of channel activity was mainly due to an increase in channel availability. Channels derived by expression of alpha1c alone were not affected by intracellular alkalosis. Our results demonstrate that the pHi sensitivity of L-type Ca2+ channels is conferred by the beta subunit of the channel complex.

References

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Citations

Jul 17, 2001·Journal of Cellular Biochemistry. Supplement·A SacchettiS Alberti
Oct 15, 2011·Protein Engineering, Design & Selection : PEDS·Laurence GuglielmiPierre Martineau
Dec 3, 2005·The Journal of Clinical Investigation·Ilona BodiArnold Schwartz
Feb 2, 2006·Journal of Vascular Research·Ichiro WakabayashiKlaus Groschner
Aug 13, 2004·American Journal of Physiology. Cell Physiology·Juan J Fraire-Zamora, Marco T González-Martínez
Mar 15, 2006·Biochemical and Biophysical Research Communications·Paloma del Carmen Neri-VidaurriMarco T González-Martínez

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