Essential role of the SycP chaperone in type III secretion of the YspP effector.

Journal of Bacteriology
Hiroyuki Matsumoto, Glenn M Young

Abstract

The Ysa type III secretion (T3S) system enhances gastrointestinal infection by Yersinia enterocolitica bv. 1B. One effector protein targeted into host cells is YspP, a protein tyrosine phosphatase. It was determined in this study that the secretion of YspP requires a chaperone, SycP. Genetic analysis showed that deletion of sycP completely abolished the secretion of YspP without affecting the secretion of other Ysps by the Ysa T3S system. Analysis of the secretion and translocation signals of YspP defined the first 73 amino acids to form the minimal region of YspP necessary to promote secretion and translocation by the Ysa T3S system. Function of the YspP secretion/translocation signals was dependent on SycP. Curiously, when YspP was constitutively expressed in Y. enterocolitica bv. 1B, it was recognized and secreted by the Ysc T3S system and the flagellar T3S system. In these cases, the first 21 amino acids were sufficient to promote secretion, and while SycP did enhance secretion, it was not essential. However, neither the Ysc T3S system nor the flagellar T3S system translocated YspP into mammalian cells. This supports a model where SycP confers secretion/translocation specificities for YspP by the Ysa T3S system. A series of...Continue Reading

References

Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D H Figurski, D R Helinski
Mar 1, 1991·Journal of Bacteriology·T Michiels, G R Cornelis
Jan 1, 1993·Methods in Enzymology·R M HortonL R Pease
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·M P SoryG R Cornelis
May 1, 1997·Molecular Microbiology·L W ChengO Schneewind
Jul 1, 1997·Molecular Microbiology·G M Young, V L Miller
May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·G M YoungV L Miller
Oct 6, 2000·Annual Review of Microbiology·G R Cornelis, F Van Gijsegem
Jan 3, 2001·Molecular Microbiology·S A LloydH Wolf-Watz
Feb 15, 2002·Journal of Bacteriology·Briana M Young, Glenn M Young
May 25, 2002·Journal of Bacteriology·Kumaran S Ramamurthi, Olaf Schneewind
Aug 7, 2002·The Journal of Cell Biology·Guy R Cornelis
Oct 3, 2002·Nature Reviews. Molecular Cell Biology·Guy R Cornelis
Mar 5, 2003·Current Opinion in Microbiology·Claude ParsotAnne-Laure Page
Mar 7, 2003·FEMS Microbiology Letters·Mario F Feldman, Guy R Cornelis
Sep 25, 2003·Nature Reviews. Molecular Cell Biology·C Erec Stebbins, Jorge E Galán
Feb 6, 2004·Molecular Microbiology·Sang Ho Lee, Jorge E Galán
Dec 14, 2004·Microbiology and Molecular Biology Reviews : MMBR·Partho Ghosh
Jan 22, 2005·Molecular Microbiology·Liliana C Losada, Steven W Hutcheson
Feb 8, 2005·Current Opinion in Microbiology·Lorena NavarroJack E Dixon
Jul 8, 2005·The Journal of Biological Chemistry·Martin LocherGottfried Wilharm

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