PMID: 8985179Dec 15, 1996Paper

Est1 has the properties of a single-stranded telomere end-binding protein

Genes & Development
V Virta-PearlmanV Lundblad

Abstract

In Saccharomyces cerevisiae, deletion of the EST1 gene results in phenotypes identical to those displayed by a deletion of a known component of telomerase (the yeast telomerase RNA), arguing that EST1 is also critical for telomerase function. In this study, we show that the Estl protein binds to yeast G-rich telomeric oligonucleotides in vitro. Binding is specific for single-stranded substrates and requires a free 3' terminus, consistent with the properties expected for a protein bound to the 3' single-stranded G-rich extension present at the telomere. Assessment of the in vivo function of this single-stranded DNA-binding protein has shown that EST1 acts in the same pathway of telomere replication as the TLC1 telomerase RNA, by several different genetic criteria: est1 tlc1 double mutant strains show no enhancement of phenotype relative to either single mutant strain, and EST1 dominant mutations have an effect on telomeric silencing similar to that displayed by TLC1 previously. We propose that Est1 is a telomere end-binding protein that is required to mediate recognition of the end of the chromosome by telomerase.

References

Jun 1, 1992·Current Opinion in Cell Biology·C M Price
Mar 25, 1992·Nucleic Acids Research·S J McKay, H Cooke
Jun 25, 1992·Nucleic Acids Research·A J Lustig
Feb 2, 1990·Science·D Shippen-Lentz, E H Blackburn
Oct 25, 1991·Nucleic Acids Research·G W Fang, T R Cech
Aug 11, 1989·Nucleic Acids Research·A M Zahler, D M Prescott
Apr 27, 1989·Nature·W R Brown
Oct 1, 1987·Genes & Development·C M Price, T R Cech
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·L A KlobutcherD M Prescott
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·A F PlutaV A Zakian
Jul 12, 1984·Nature·J ShampayE H Blackburn
Dec 20, 1982·Nucleic Acids Research·A F PlutaB B Spear
Dec 8, 1995·Science·V A Zakian
Jan 4, 1994·Proceedings of the National Academy of Sciences of the United States of America·D E ShippenC M Price
Sep 1, 1995·Science·J FengJ Yu
Oct 21, 1994·Science·M S Singer, D E Gottschling
Jul 21, 1995·Science·M Cohn, E H Blackburn
Aug 3, 1995·Nature·M J McEachern, E H Blackburn
Sep 15, 1995·The Journal of Biological Chemistry·N F Lue, J C Wang
Jun 21, 1995·Journal of the National Cancer Institute·G B Morin
Apr 14, 1995·The Journal of Biological Chemistry·L HarringtonC Greider
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·L M KonkelJ Berman
Jun 15, 1994·Genes & Development·J B Stavenhagen, V A Zakian
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·C M CounterC B Harley
Feb 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K R ProwseC W Greider
Apr 2, 1996·Proceedings of the National Academy of Sciences of the United States of America·B R SteinerB Futcher
May 3, 1996·Cell·R J WellingerV A Zakian
Jan 1, 1996·Annual Review of Biochemistry·C W Greider

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Citations

Sep 1, 2005·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Lea Harrington
May 22, 2003·Cancer Letters·Lea Harrington
Jun 6, 2003·Current Opinion in Cell Biology·Andrew K P Taggart, Virginia A Zakian
Jul 8, 1999·Current Opinion in Cell Biology·T M Bryan, T R Cech
Feb 19, 1999·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·G T HuangJ C Sheu
Aug 1, 1997·Trends in Cell Biology·C BlueE Gilson
Jan 10, 2013·Nature Reviews. Molecular Cell Biology·Jayakrishnan Nandakumar, Thomas R Cech
Jan 26, 2010·Nature Structural & Molecular Biology·Ming-Liang ZhangJin-Qiu Zhou
Jul 25, 2006·Nature Structural & Molecular Biology·Jennifer L OsterhageKatherine L Friedman
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Diane C DeZwaan, Brian C Freeman
Oct 5, 2011·Proceedings of the National Academy of Sciences of the United States of America·Yun Wu, Virginia A Zakian
Nov 15, 1997·Molecular Biology of the Cell·X Fan, C M Price
Jun 16, 1999·The EMBO Journal·S MarcandE Gilson
Jul 25, 2000·Nucleic Acids Research·K FörstemannJ Lingner
Nov 14, 1997·Genes & Development·J Prescott, E H Blackburn
May 23, 2003·Cold Spring Harbor Symposia on Quantitative Biology·M L DuBoisD E Gottschling
Jun 15, 2007·Molecular and Cellular Biology·Eun Young YuNeal F Lue
Jan 12, 2011·Molecular and Cellular Biology·Xia-Jing TongJin-Qiu Zhou
Oct 25, 2000·Molecular and Cellular Biology·N GrandinM Charbonneau
Jan 11, 2000·Molecular and Cellular Biology·A Adams MartinC Holm
Feb 25, 2000·Molecular and Cellular Biology·J ZhouB Futcher
Aug 9, 2002·Molecular and Cellular Biology·Francisco FerrezueloBruce Futcher
Mar 9, 2002·Molecular and Cellular Biology·April J LivengoodThomas R Cech

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