Established cell lines and primary cultures in testing medical devices in vitro

Toxicology in Vitro : an International Journal Published in Association with BIBRA
E CenniA Pizzoferrato

Abstract

In testing for the cytotoxicity of medical devices, crucial parameters are the type of cells, the duration of the exposure, the physical form of the device and the method of evaluation. Using cell cultures the changes of cell functions induced by artificial materials may be evaluated. In screening tests the effect of biomaterials on functions common to all cell types is investigated through both continuous lines and primary cultures. In supplementary tests the effect of biomaterials on specific functions is studied, employing cells of the same type of those which will be in contact with the device in vivo. Osteoblasts, either from primary culture or an established line, are used to study the interaction with materials for bone implants. We evaluated whether dental cements affect the replication cycle of osteoblast-like cells MG63. The S phase was inhibited by the cements with a phenolic group, even though to a different extent. Mononuclear cell cultures are used to investigate the immune response, which is important in the host reaction to implant. We investigated whether the chromium ions released in a biological fluid may induce PBMC to produce cytokines involved in bone resorption. Chromium extract did not impair TNFalpha re...Continue Reading

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Citations

Aug 30, 2005·Yonsei Medical Journal·Hyun Sook BaekJong-Chul Park
Jun 12, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Kamal Hany HusseinHeung-Myong Woo
May 3, 2012·Experimental Biology and Medicine·Shu-Juan YuGuo-Xiong Zhu
Dec 1, 2001·Human & Experimental Toxicology·A B PantP K Seth
Feb 14, 2009·Biomedical Instrumentation & Technology·Anne D LucasDiane Wray-Cahen
Feb 17, 2021·Biomedical Materials·Uzair AhmedAnwarul Hasan

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