Established method of chondroitin sulphate extraction from buffalo (Bubalus bubalis ) cartilages and its identification by FTIR

Journal of Food Science and Technology
G SundaresanMahantesh F Meti

Abstract

A study was conducted for extraction of chondroitin sulphate (CS) from buffalo tracheal, nasal and joint cartilages. CS was extracted from cartilages using 0.25% papain digestion, dialyzed, precipitated with 10% TCA and finally lyophilized to dry powder. Dimethylmethylene blue assay was performed to estimate the quantity of CS extracted. Identification of extracted CS was performed with SDS-PAGE and Fourier transforms infrared spectroscopy (FTIR). SDS-PAGE analysis of extracted CS revealed similar electrophoretic pattern to that of standard and the molecular weight ranged from 5 to 20 kDa. FTIR spectra of extracted CS revealed presence of characteristic peaks of -CONH vibration of amide group, coupling of C-O stretching vibration, S=O stretching vibrations and -C-O-S molecules confirms the CS moiety. It can be concluded that extraction method adopted could efficiently be utilized for the extraction of CS from buffalo by-products like tracheal, nasal and joint cartilages.

References

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Aug 10, 2000·Journal of Periodontal Research·L A RochaL C Silva
Aug 7, 2002·Poultry Science·X M LuoR M Leach
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Jun 4, 2010·Applied Microbiology and Biotechnology·Chiara SchiraldiMario De Rosa

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Citations

Jun 14, 2020·Carbohydrate Polymers·Maha M AbdallahMaria do Rosário Bronze

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BETA
electrophoresis

Software Mentioned

Image Lab

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