Establishing a mucosal gut microbial community in vitro using an artificial simulator

PloS One
LinShu LiuPeggy M Tomasula

Abstract

The Twin Simulator of the Human Intestinal Microbial Ecosystem (TWINSHIME®) was initially developed to study the luminal gut microbiota of the ascending (AC), transverse (TC), and descending (DC) colon regions. Given the unique composition and potential importance of the mucosal microbiota for human health, the TWINSHIME was recently adapted to simulate the mucosal microbiota as well as the luminal community. It has been previously demonstrated that the luminal community in the TWINSHIME reaches a steady state within two weeks post inoculation, and is able to differentiate into region specific communities. However, less is known regarding the mucosal community structure and dynamics. During the current study, the luminal and mucosal communities in each region of the TWINSHIME were evaluated over the course of six weeks. Based on 16S rRNA gene sequencing and short chain fatty acid analysis, it was determined that both the luminal and mucosal communities reached stability 10-20 days after inoculation, and remained stable until the end of the experiment. Bioinformatics analysis revealed the formation of unique community structures between the mucosal and luminal phases in all three colon regions, yet these communities were similar...Continue Reading

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Citations

Sep 4, 2019·The ISME Journal·Kaitlyn OliphantEmma Allen-Vercoe
Jun 27, 2019·American Journal of Primatology·Jenni FirrmanWilliam Rinaldi
Oct 22, 2020·Applied Microbiology and Biotechnology·Charlotte DeschampsStéphanie Blanquet-Diot
Jul 31, 2020·The Science of the Total Environment·Jannike L KrauseMartin von Bergen
Dec 24, 2019·Journal of Agricultural and Food Chemistry·Karley K MahalakLin Shu Liu
Mar 7, 2021·Pathogens·Martina FranzinGabriele Stocco
Mar 26, 2021·Applied Microbiology and Biotechnology·Jenni FirrmanPieter Van den Abbeele
Mar 28, 2021·Mucosal Immunology·Noëmie DanielBenoit Chassaing
May 10, 2020·Chemical Research in Toxicology·Angela A M KämpferRoel P F Schins
Nov 7, 2019·Journal of Agricultural and Food Chemistry·Jenni FirrmanBradley Evans
Dec 10, 2020·Food Research International·Pauline BondueVéronique Delcenserie

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Methods Mentioned

BETA
16S sequencing

Software Mentioned

Twin Simulator
QIIME
TWINSHIME
ProDigest
SHIME

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