Establishing and maintaining sequestration of Dam target sites for phase variation of agn43 in Escherichia coli.

Journal of Bacteriology
Renata Kaminska, Marjan W van der Woude

Abstract

Phase variation of the outer membrane protein Ag43 encoded by agn43 in Escherichia coli is controlled by an epigenetic mechanism. Sequestration of the regulatory region from Dam-dependent methylation has to be established and maintained throughout a generation to obtain and maintain the OFF phase. This work shows that hemimethylated DNA, which is formed by the passage of the DNA replication fork in an ON-phase cell, can be sequestered from methylation by OxyR binding, which is thus a key event for the switch from ON to OFF. No evidence was found that the protein SeqA, which also binds to the region, is involved in sequestration. To facilitate the dissection of this process further, a novel approach was introduced that does not alter the sequence of the regulatory region or the cellular concentration of Dam or OxyR, which consists of inserting auxiliary OxyR binding sites upstream of the regulatory region. Using this strategy, it was shown that the ON-to-OFF switch frequency can be modulated without changing the OFF-to-ON frequency. The data support a model in which in an ON-phase cell, the subcellular OxyR availability at the replication fork as it passes through the agn43 regulatory region is key for initiating an ON-to-OFF sw...Continue Reading

References

May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·B A BraatenD A Low
Jan 11, 1980·Nucleic Acids Research·B BowenH Weintraub
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Apr 26, 2002·Molecular Microbiology·Jason CorrentiMarjan van der Woude
Mar 20, 2003·Journal of Bacteriology·Anu WallechaMarjan van der Woude
Jun 26, 2003·The Journal of Biological Chemistry·Joo Seok HanDeog Su Hwang
May 21, 2004·The Journal of Biological Chemistry·Joo Seok HanDeog Su Hwang
Jul 3, 2004·Advances in Experimental Medicine and Biology·Aaron HerndayDavid Low
Jul 20, 2004·Clinical Microbiology Reviews·Marjan W van der Woude, Andreas J Bäumler
Sep 28, 2004·Biotechnology and Bioengineering·Laura R JarboeJames C Liao
Mar 31, 2005·Current Opinion in Genetics & Development·Jose M G Vilar, Leonor Saiz
Mar 10, 2006·Journal of Molecular Biology·John R HortonXiaodong Cheng
Sep 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Josep Casadesús, David Low
Jan 16, 2007·Nature Genetics·Han N Lim, Alexander van Oudenaarden
Nov 3, 2007·Biological Chemistry·Victor V ZinovievStanley Hattman
Apr 9, 2008·Current Opinion in Microbiology·David A Low, Josep Casadesús
May 27, 2008·The Journal of Biological Chemistry·Stephanie R Coffin, Norbert O Reich
Aug 19, 2008·Journal of Molecular Biology·Stacey N Peterson, Norbert O Reich
Sep 13, 2008·Annual Review of Microbiology·Marjan W van der Woude, Ian R Henderson
Jan 30, 2009·Protein Science : a Publication of the Protein Society·Gwendowlyn S KnappJames C Hu
Mar 4, 2009·Plasmid·Torsten Waldminghaus, Kirsten Skarstad
Mar 24, 2009·Nucleic Acids Research·Yu Seon ChungAlba Guarné
Apr 18, 2009·Genes to Cells : Devoted to Molecular & Cellular Mechanisms· MorigenKirsten Skarstad
May 8, 2009·The Journal of Biological Chemistry·Stephanie R Coffin, Norbert O Reich

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Citations

Mar 19, 2013·Journal of Bacteriology·Ankur B DaliaAndrew Camilli
Apr 19, 2011·Current Opinion in Microbiology·Dominik SatoryChristophe Herman
Feb 5, 2011·Current Opinion in Microbiology·Marjan W van der Woude
Apr 18, 2013·The Journal of Biological Chemistry·Josep Casadesús, David A Low
Sep 15, 2021·Epigenetics : Official Journal of the DNA Methylation Society·Matthieu BruneauxTarmo Ketola

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