Abstract
The authors established apoptosis resistant COS-1, myeloma, hybridoma, and Friend leukemia cell lines by genetically engineering cells, aiming at more efficient protein production by cell culture. COS-1 cells, which are most widely used for eukariotic gene expression, were transfected with human bcl-2 gene. Both bcl-2 and mock transfected COS-1 cells were cultured at low (0.2%) serum concentration for 9 days. The final viable cell number of the bcl-2 transfected cells was ninefold of that of the mock transfectants. Both bcl-2 and mock transfectants were further transfected with the vector pcDNA-λ containing SV40 ori and immunoglobulin λ gene for transiently expressing λ protein. The bcl-2 expressing COS-1 cells produced more λ protein than the mock transfected COS-1 cells after 4 days posttransfection.Mouse myeloma p3-X63-Ag.8.653 cells, which are widely used as the partner for preparing hybridoma, and hybridoma 2E3 cells were transfected with human bcl-2 gene. Both bcl-2 transfected myeloma and hybridoma survived longer than the corresponding original cells in batch culture. The bcl-2 transfected 2E3 cells survived 2 to 4 four days longer in culture, producing 1.5- to 4-fold amount of antibody in comparison with the mock trans...Continue Reading
References
May 1, 1990·Biotechnology Progress·E Suzuki, D F Ollis
Jan 1, 1992·Cytotechnology·F MakishimaE Suzuki
Sep 1, 1990·The Journal of Experimental Medicine·H KarasuyamaF Melchers
Dec 5, 1990·Journal of Immunological Methods·D DuvalA Marcadet
Feb 1, 1990·The Journal of Experimental Medicine·N TohyamaT Tada
Sep 29, 1988·Nature·D L VauxJ M Adams
Dec 1, 1981·Cell·P MellonT Maniatis
Jan 1, 1994·Cytotechnology·K TakahashiE Suzuki
Jan 27, 1995·Cell·S TakayamaJ C Reed
Jan 1, 1993·Immunology Letters·T Vomastek, F Franĕk
Aug 5, 1988·Biotechnology and Bioengineering·M W GlackenA J Sinskey
Sep 5, 1994·Biotechnology and Bioengineering·R P SinghA N Emery
Nov 5, 1994·Biotechnology and Bioengineering·S Mercille, B Massie
Oct 20, 1995·Biotechnology and Bioengineering·Y ItohE Suzuki
Jan 1, 1995·Cytotechnology·F Franěk, K Chládková-Šrámková
Citations
Jul 9, 1999·Biotechnology and Bioengineering·S Mercille, B Massie
Jan 29, 2000·Biotechnology and Bioengineering·A J MastrangeloM J Betenbaugh
Nov 13, 2008·Cytotechnology·Nyaradzo T Mukwena, Mohamed Al-Rubeai
Aug 28, 1998·Trends in Biotechnology·A J Dickson
Nov 1, 1997·Cytotechnology·T FujitaE Suzuki
Nov 13, 2008·Cytotechnology·A HiguchiM Hara
Nov 13, 2008·Cytotechnology·S Mercille, B Massie
Nov 13, 2008·Cytotechnology·D FassnachtR Pörtner
Feb 10, 1999·Biotechnology and Bioengineering·J GoswamiD I Wang
Apr 1, 1999·Biotechnology and Bioengineering·N H SimpsonM Al-Rubeai
Dec 11, 2002·Biotechnology and Bioengineering·Tina M SauerwaldMichael J Betenbaugh
May 15, 2007·Biotechnology and Bioengineering·Mike MolletJeffrey J Chalmers
Apr 7, 2006·Biotechnology and Bioengineering·Tina M SauerwaldMichael J Betenbaugh
Feb 27, 2004·Cell Biology International·Xian-Hui WangZhi-nan Chen
Oct 9, 2007·Biotechnology Advances·Era Jain, Ashok Kumar