Establishing, Expanding, and Certifying a Closed Colony of Phlebotomus argentipes (Diptera: Psychodidae) for Xenodiagnostic Studies at the Kala Azar Medical Research Center, Muzaffarpur, Bihar, India

Journal of Medical Entomology
Puja TiwaryP G Lawyer

Abstract

This pilot project was preliminary and essential to a larger effort to define the ability of certain human-subject groups across the infection spectrum to serve as reservoirs of Leishmania donovani infection to sand flies in areas of anthroponotic transmission such as in Bihar state, India. This is possible only via xenodiagnosis of well-defined subject groups using live vector sand flies. The objective was to establish at the Kala Azar Medical Research Center (KAMRC), Muzaffarpur, Bihar, India, a self-sustaining colony of Phlebotomus argentipes (Annandale & Brunneti), closed to infusion with wild-caught material and certified safe for human xenodiagnosis. Prior to this endeavor, no laboratory colony of this vector existed in India meeting the stringent biosafety requirements of this human-use study. From March through mid-December, 2015, over 68,000 sand flies were collected in human dwellings and cattle sheds using CDC-type light traps over 254 nights. Blood-fed and gravid P. argentipes females were selected and placed individually in isoline-rearing vials for oviposition, and >2,500 egg clutches were harvested. Progeny were reared according to standard methods, providing a continuous critical mass of F1 males and females to ...Continue Reading

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Citations

Nov 16, 2017·Parasite : Journal De La Société Française De Parasitologie·Phillip LawyerPetr Volf
Oct 6, 2018·Expert Review of Anti-infective Therapy·Shyam SundarJaya Chakravarty
Aug 14, 2020·PLoS Neglected Tropical Diseases·Om Prakash SinghShyam Sundar
Dec 5, 2018·BMC Veterinary Research·Shuai HanCan-Jun Zheng
Jun 21, 2018·Molecular Diagnosis & Therapy·Shyam Sundar, Om Prakash Singh
Jun 4, 2018·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Epke A Le RutteSake J de Vlas
Mar 27, 2021·Frontiers in Cellular and Infection Microbiology·Rajesh GarlapatiShaden Kamhawi
Apr 13, 2021·Frontiers in Cellular and Infection Microbiology·Shuai HanWei-Ping Wu
Mar 10, 2021·Transboundary and Emerging Diseases·Anurag Kumar KushwahaChristine A Petersen

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