Establishing intracranial brain tumor xenografts with subsequent analysis of tumor growth and response to therapy using bioluminescence imaging.

Journal of Visualized Experiments : JoVE
Tomoko Ozawa, C David James

Abstract

Transplantation models using human brain tumor cells have served an essential function in neuro-oncology research for many years. In the past, the most commonly used procedure for human tumor xenograft establishment consisted of the collection of cells from culture flasks, followed by the subcutaneous injection of the collected cells in immunocompromised mice. Whereas this approach still sees frequent use in many laboratories, there has been a significant shift in emphasis over the past decade towards orthotopic xenograft establishment, which, in the instance of brain tumors, requires tumor cell injection into appropriate neuroanatomical structures. Because intracranial xenograft establishment eliminates the ability to monitor tumor growth through direct measurement, such as by use of calipers, the shift in emphasis towards orthotopic brain tumor xenograft models has necessitated the utilization of non-invasive imaging for assessing tumor burden in host animals. Of the currently available imaging methods, bioluminescence monitoring is generally considered to offer the best combination of sensitivity, expediency, and cost. Here, we will demonstrate procedures for orthotopic brain tumor establishment, and for monitoring tumor gro...Continue Reading

References

Jun 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Kevin CamphausenPhilip J Tofilon
Dec 21, 2007·Brain Pathology·Gilson S BaiaAnita Lal
Jul 9, 2009·Journal of Neuro-oncology·Rintaro HashizumeNalin Gupta

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Citations

Jan 11, 2012·Journal of Neuro-oncology·Yasuyuki AokiNalin Gupta
Sep 4, 2013·The Journal of Clinical Investigation·Grit S Herter-SprieKwok-Kin Wong
Jan 23, 2013·Molecular and Cellular Neurosciences·Navjot KaurAnjali Shiras
Jun 27, 2015·Proceedings of the National Academy of Sciences of the United States of America·Chiara PastoriClaes Wahlestedt
Mar 16, 2013·British Journal of Pharmacology·A C O'FarrellJ H Gill
Mar 31, 2015·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Rodney B LuworAndrew H Kaye
Feb 24, 2015·Magnetic Resonance Imaging·Rossana RitelliPasquina Marzola
Apr 5, 2017·Proceedings of the National Academy of Sciences of the United States of America·Timothy L SitaAlexander H Stegh
Jan 11, 2017·Journal of Experimental & Clinical Cancer Research : CR·Irene YlivinkkaMarko Hyytiäinen
Apr 24, 2017·Journal of Neuro-oncology·Ilya V UlasovCharles Cobbs
Mar 7, 2021·International Journal of Molecular Sciences·Jun NakayamaKentaro Semba

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Methods Mentioned

BETA
xenografts
bioluminescence imaging
xenograft

Software Mentioned

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