Establishing multiple osteogenic differentiation pathways of mesenchymal stem cells through different scaffold configurations.

Journal of Tissue Engineering and Regenerative Medicine
Salima NedjariGeorge Altankov

Abstract

Mimicking the complex organization of the extracellular matrix (ECM), especially its structure and dimensionality, is necessary to produce living tissues from stem cells. In compliance with a previously established role of nanofiber organization for the osteogenic differentiation of stem cells, here we used hybrid fibrinogen/poly(l-lactide-ε-caprolactone) (FBG/PLCL) nanofibers arranged in aligned and honeycomb configurations, to recapitulate the highly oriented ECM of the cortical bone and the sponge-like (i.e., honeycomb) environment of the cancellous one, respectively. Using special bilayered constructs, we demonstrate that the dimensionality (i.e., 2D vs. 3D) of the nanofibers as well as their architecture (i.e., honeycomb vs. aligned) affects differently the overall morphology and the expression of multiple osteogenic genes of human adipose-derived mesenchymal stem cells (ADMSCs). The cells had elongated shape with markedly increased cell mobility when seeded on aligned nanofibers. Conversely, on honeycomb-shaped nanofibers, ADMSCs initially concentrated inside the honeycomb curvatures adopting rounded morphology, but late, they formed network-like structures overlaying the honeycomb curvatures. By employing quantitative po...Continue Reading

References

Feb 1, 1978·Journal of Dental Research·P K YenJ H Shaw
Sep 1, 1990·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J T NinomiyaK G Mann
Jun 1, 1985·Collagen and Related Research·A L BronckersW T Butler
Jan 1, 1985·Journal of Biomechanics·L J Gibson
Aug 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R Raghow
Aug 1, 1996·Nature·P DucyG Karsenty
Apr 12, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·B GanssJ Sodek
Sep 1, 2000·Osteoarthritis and Cartilage·A M DeLiseR S Tuan
Dec 6, 2003·Circulation Research·V Planat-BénardL Casteilla
Oct 7, 2004·Macromolecular Bioscience·António J SalgadoRui L Reis
Jun 23, 2005·Frontiers in Bioscience : a Journal and Virtual Library·Ton LismanPhilip G de Groot
Oct 11, 2005·Annual Review of Cell and Developmental Biology·Linheng Li, Ting Xie
Aug 28, 2007·International Journal of Nanomedicine·Rajesh Vasita, Dhirendra S Katti
May 7, 2008·The Journal of Experimental Medicine·Luc MalavalJane E Aubin
Sep 10, 2008·Acta Biomaterialia·Moncy V JoseElijah Nyairo
Nov 15, 2008·Clinical Journal of the American Society of Nephrology : CJASN·Bart Clarke
Dec 31, 2008·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Wook-Young BaekJung-Eun Kim
Dec 2, 2009·Advances in Experimental Medicine and Biology·Toshihisa Komori
Feb 22, 2012·Journal of Biomedical Materials Research. Part a·You-Zhi CaiXiao-Hui Zou
Dec 12, 2012·Journal of Cellular Biochemistry·Krishna M Sinha, Xin Zhou
Jan 24, 2013·Critical Reviews in Biomedical Engineering·Ami R AminiSyam P Nukavarapu
Jun 1, 2013·Materials Today·Xianfeng WangBingyun Li
Aug 21, 2014·Macromolecular Bioscience·Salima NedjariGuy Schlatter
Mar 16, 2017·Journal of Biomedical Materials Research. Part a·Dencho GugutkovGeorge Altankov

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Citations

Aug 16, 2021·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Zhenghai ZhangYan Liu

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