Establishing the pangolin mitochondrial D-loop sequences from the confiscated scales

Forensic Science International. Genetics
Hsing-Mei HsiehLi-Chin Tsai

Abstract

Pangolin scales are encountered in traditional East Asian medicines (TEAM) and the ever increasing demand for these scales has escalated the decline in the numbers of these mammals. The identification of protected pangolin species is necessary to enforce international and national legislation as well as assist with conservation measures. There is limited morphological feature on a pangolin scale thus requiring DNA analysis as a means of identification. We report on the isolation of DNA from pangolin scales and a strategy for obtaining the full length of the mitochondrial D-loop, being 1159 bp. Primer sets creating five overlapping amplicons were designed to amplify sections of this mitochondrial DNA locus. DNA from the blood stain of nineteen Formosan pangolins (Manis pentadactyla pentadactyla) along with 145 scale samples that were suspected to have come from pangolins, was amplified and sequenced; leading to a total of 91 D-loop sequences being obtained. The 19 Formosan pangolin sequences produced 5 haplotypes and 72 of the 145 seized scales provided useable sequence classified as a further 38 haplotypes. The D-loop sequences from those scales suspected to be from a pangolin had a higher similarity to any of the 19 samples ta...Continue Reading

References

Oct 1, 1996·Journal of Molecular Evolution·M S Springer, E Douzery
Jul 3, 1997·Nature·M S SpringerM J Stanhope
Feb 24, 2001·Nature·W J MurphyS J O'Brien
May 18, 2001·International Journal of Legal Medicine·A HellmannM Wittig
May 30, 2001·Forensic Science International : Synergy·L C TsaiW Goodwin
Sep 13, 2003·Forensic Science International : Synergy·Hsing-Mei HsiehJames Chun-I Lee
Aug 1, 1951·The Journal of Comparative Neurology·R SNIDER, E ELDRED
Jun 28, 2005·Forensic Science International : Synergy·Hirokazu MatsudaNobuhiro Yukawa
Jan 21, 2006·Journal of Forensic Sciences·Hsing-Mei HsiehJames Chun-I Lee
Mar 7, 2006·PLoS Biology·Jan Ole KriegsJürgen Schmitz
Jul 15, 2006·Forensic Science International : Synergy·S AmoryB Ludes
Jul 18, 2006·International Journal of Legal Medicine·Takashi KitanoMotoki Osawa
Aug 5, 2006·Electrophoresis·James Chun-I LeeHsing-Mei Hsieh
Aug 26, 2006·Forensic Science International : Synergy·Matthew Phipps, Susan Petricevic
Jul 21, 2007·Nature Protocols·Nadin Rohland, Michael Hofreiter
Jul 10, 2008·International Journal of Legal Medicine·James Chun-I LeeLi-Chin Tsai
May 1, 2007·Zoo Biology·Ci Wen YangEllen S Dierenfeld

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Citations

Apr 1, 2014·Forensic Science International. Genetics·Rebecca N JohnsonAdrian Linacre
Feb 9, 2017·Genome Génome / Conseil National De Recherches Canada·Monica MwaleAntoinette Kotzé
Oct 18, 2016·Mitochondrial DNA. Part B, Resources·Adrian U LuczonIan Kendrich C Fontanilla
Jan 19, 2021·Mitochondrial DNA. Part B, Resources·Sandeep ShresthaSanjay Nath Khanal
May 1, 2021·Genes·Adrian Linacre
Apr 27, 2021·Forensic Science International : Synergy·Prajnashree PriyambadaMukesh Thakur
Sep 4, 2021·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Chunbing LiuLi Yu

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