Establishment and Application of Flavivirus Replicons

Advances in Experimental Medicine and Biology
Beate Mareike Kümmerer

Abstract

Dengue virus (DENV) and Zika virus (ZIKV) are enveloped, positive-strand RNA viruses belonging to the genus Flavivirus in the family Flaviviridae. The genome of ~11 kb length encodes one long open reading frame flanked by a 5' and a 3' untranslated region (UTR). The 5' end is capped and the 3' end lacks a poly(A) tail. The encoded single polyprotein is cleaved co-and posttranslationally by cellular and viral proteases. The first one-third of the genome encodes the structural proteins (C-prM-E), whereas the nonstructural (NS) proteins NS1-NS2A-NS3-NS4A-2K-NS4B-NS5 are encoded by the remaining two-thirds of the genome.Research on flaviviruses was driven forward by the ability to produce recombinant viruses using reverse genetics technology. It is known that the purified RNA of flaviviruses is per se infectious, which allows initiation of a complete viral life cycle by transfecting the genomic RNA into susceptible cells. In 1989, the first infectious flavivirus RNA was transcribed from full-length cDNA templates of yellow fever virus (YFV) facilitating molecular genetic analyses of this virus. In addition to the production of infectious recombinant viruses, reverse genetics can also be used to establish non-infectious replicons. R...Continue Reading

Citations

Aug 29, 2018·Viruses·Juan-Carlos SaizMiguel A Martín-Acebes
Oct 1, 2020·Viruses·Coleman Baker, Pei-Yong Shi
Apr 4, 2021·Vaccines·Judith Estévez-HerreraAgustín Valenzuela-Fernández
Jul 3, 2021·Life·Rong ZhaoJimin Cao
Aug 14, 2021·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Noémie OechslinJérôme Gouttenoire

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