PMID: 7541602Jul 1, 1995Paper

Establishment and characteristics of a gastric cancer cell line (HuGC-OOHIRA) producing high levels of G-CSF, GM-CSF, and IL-6: the presence of autocrine growth control by G-CSF

American Journal of Hematology
M BabaK Tani

Abstract

We successfully established a human gastric cancer cell line, HuGC-OOHIRA, from the ascites of a 60-year-old patient with advanced gastric cancer (poorly differentiated adenocarcinoma) complicated by peritonitis carcinomatosa and leukocytosis of unknown origin. Morphologically, the cells were polygonal and adhered weakly to the culture flask. They tended to pile up upon reaching confluence. Chromosome analysis revealed that the cell line has two modes of chromosome number, namely near diploidy and tetraploidy. Double minutes (DMs) were present in abundance in each cell. The doubling time was 25-30 hr. The cell line was successfully transplanted into nude mice, and their peripheral leukocyte counts increased in proportion to the growth of the tumors. At 2 weeks after the transplantation, the serum rG-CSF level was elevated to 2,893 pg/ml. The concentration of human G-CSF in the culture supernatants was an extraordinary high level of 145,380 pg/ml/day. Secretion of GM-CSF and IL-6 was also detected. The intracellular localization of the G-CSF was identified for the first time by immunofluorescence. Moreover, Northern blot analysis detected G-CSF mRNA in this cell line. Anti-recombinant human G-CSF serum suppressed the propagation...Continue Reading

References

Dec 1, 1990·Gastroenterologia Japonica·T TakedaT Miyaji

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Citations

Aug 9, 2005·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Taku YokoyamaKen Saitoh
Nov 28, 2002·Biological Chemistry·Lars Hareng, Thomas Hartung
Dec 4, 2004·Clinical Chemistry and Laboratory Medicine : CCLM·Barbara Mroczko, Maciej Szmitkowski
Nov 25, 2003·Cancer Investigation·Chi-Kuan Ho, Chieh-Chih Chen
May 23, 2003·The Journal of International Medical Research·K MatsuoS Kohno
Oct 26, 2016·World Journal of Clinical Oncology·Hiroaki NagataHiroshi Konosu
Dec 31, 2010·Anatomy & Cell Biology·Sung-Min AhnBonghee Lee
Jul 19, 2013·Gan to kagaku ryoho. Cancer & chemotherapy·Hiroto SaitoTetsuo Ohta
Sep 2, 1998·International Journal of Cancer. Journal International Du Cancer·K G SteubeH G Drexler
Dec 22, 1999·International Journal of Cancer. Journal International Du Cancer·M M Mueller, N E Fusenig
Nov 1, 2017·Journal of Pancreatic Cancer·Evan S Wu, Samer A Srour

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