Establishment and characterization of a human-papillomavirus negative, p53-mutation negative human cervical cancer cell line

Cancer Letters
C Y ChouT M Chen

Abstract

A human cervical cancer cell line, CX, was established from a patient with squamous cell carcinoma of the uterine cervix. The CX cells were epithelial in morphology with relatively large vesicular nuclei, and prominent nucleoli. Cytoplasmic organelles were generally sparse but tonofilaments were relatively abundant. The cells grew as a compact sheet with close membrane approximation interconnected by desmosome-like junctions. CX cells contained cytokeratin, but not vimentin. Elevated levels of squamous cell carcinoma antigen and carcinoembryonic antigen were detected in the cell supernatants. Population doubling time was estimated to be about 20 h. CX cells were not able to grow in soft agar and not tumorigenic in nude mice. Chromosome analysis revealed that CX cells were heterogeneous and mainly had a female diploid karyotype. Unlike cervical cancer cell lines published previously, CX cells were demonstrated to be human papillomavirus-negative, p53 mutation-negative. Based on the distinct characteristics, CX cell line may prove to be a useful tool for the study of human cervical carcinogenesis.

References

Nov 11, 1992·International Journal of Cancer. Journal International Du Cancer·F X BoschL C Gonzalez
May 30, 1992·Lancet·R M Busby-EarleC C Bird
May 14, 1992·The New England Journal of Medicine·A J Levine
Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M ScheffnerP M Howley
Jul 5, 1991·Science·M HollsteinC C Harris
Jun 6, 1991·Nature·A J LevineC A Finlay
Sep 1, 1990·Journal of Occupational Medicine. : Official Publication of the Industrial Medical Association·C R Smart
Feb 1, 1990·Cancer Genetics and Cytogenetics·N B AtkinM F Fox
Oct 30, 1971·Lancet·M Seabright
Apr 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S M HsuH Fanger
Apr 15, 1994·International Journal of Cancer. Journal International Du Cancer·T M ChenC Y Hsieh

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Citations

Dec 10, 2003·Obstetrics and Gynecology·Keng-Fu HsuCheng-Yang Chou
Apr 3, 2009·Cancer Letters·Tatjana MijatovicWalter Berger
Mar 17, 2012·Reproductive Sciences·Ya-Min ChengMing-Jenn Chen
Oct 31, 2020·Experimental and Molecular Pathology·Yuanlin LiuYanling Wu

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