Establishment and characterization of a new human acute myelomonocytic leukemia cell line JIH-3

Leukemia Research
Jinlan PanJuan Shen

Abstract

Here, a new acute myelomonocytic leukemia (AMML) cell line, JIH-3, is reported, and its biological characteristics are described. JIH-3 cells were maintained without any cytokines for 27 months. The JIH-3 cell line showed typical myelomonocytic morphological features. Additionally, it mainly expressed myeloid and monocytic markers (CD13, CD14, CD11b, CD15 and CD33), although it also expressed other antigens such as the markers of T and B lymphocytic lineage as well as stem cell, progenitor cell, and natural killer cell-related antigens (CD4, CD5, CD7, CD10, CD22, CD34, CD38, HLADR, CD16/CD56 and CD56); the expression of these markers, suggested that this cell line was in the early stage of myelomonocytic differentiation. Cytogenetic analysis initially showed a karyotype of 46, XY, del(7) (p1?3p2?2). During the passage period, the cells with this karyotype gradually decreased and were replaced by cells with a 45,XY,dic(4;7)(p11;p11),del(15)(q2?2) karyotype. Chromosome painting showed a deletion in the short arm of chromosome 7 for del(7)(p1?3p2?2) and der(4;7)(p11;p11). The latter had larger deleted segment than the former. Fluorescence in situ hybridization (FISH) revealed the dicentric nature of der(4;7), and Multiplex FISH (M...Continue Reading

References

Mar 1, 1997·Blood Reviews·E Johnson, F E Cotter
Mar 1, 2006·Genes, Chromosomes & Cancer·Tianyu LiJinlan Pan
Aug 30, 2008·British Journal of Haematology·Tobias BenthausAnnika Dufour
Mar 10, 2010·Experimental Cell Research·Martin Jädersten, Eva Hellström-Lindberg
Dec 7, 2010·Best Practice & Research. Clinical Haematology·Benjamin L Ebert

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