Establishment and characterization of a new, spontaneously immortalized, pancreatic ductal cell line from the Syrian golden hamster

Cell and Tissue Research
T TakahashiP M Pour

Abstract

Spontaneously immortal pancreatic cell lines are not available. By use of a defined culture medium, such a line (TAKA-1) was established from the Syrian golden hamster. Cytological, cytogenetic, molecular biological, enzymatic and receptor patterns as well as antigenicity were studied and were compared with those of the normal hamster pancreatic ductal cells in vivo. TAKA-1 cells grew exponentially in a monolayer on collagen gel in a defined medium but did not proliferate in soft agar. Ultrastructurally, the cells closely resembled the normal hamster pancreatic ductal cells. Similarities and dissimilarities were found between the normal ductal cells and TAKA-1 cells. Similarities included the presence of cytokeratin, carbonic anhydrase and some tumor-associated antigens. However, unlike the normal ductal cells, TAKA-1 cells expressed blood group A antigen and anti-vimentin, showed affinity to selected lectins, and an abnormality of chromosome 3, which is suggested to be associated with immortality. Moreover, unlike the hamster pancreatic ductal cancer cells but like the normal hamster pancreatic ductal cells, TAKA-1 cells did not have a c-Ki-ras mutation. EGF, TGF-alpha and secretin, but not CCK or GRP, bound to the TAKA-1 cell...Continue Reading

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Citations

Aug 18, 2012·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·S A MüllerA Ulrich
Nov 10, 1998·International Journal of Cancer. Journal International Du Cancer·I ToshkovP M Pour
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May 29, 2000·Pancreas·B M SchmiedP M Pour
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Nov 14, 2002·Laboratory Investigation; a Journal of Technical Methods and Pathology·Irene EspositoHelmut Friess
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Mar 31, 1998·International Journal of Pancreatology : Official Journal of the International Association of Pancreatology·Y MoritaI Makino

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