Establishment and characterization of a novel cell line from the brain of golden pompano (Trachinotus ovatus)

In Vitro Cellular & Developmental Biology. Animal
Pengfei LiQiwei Qin

Abstract

Golden pompano is a commercially important marine fish that is widely cultured in China, Japan, and Southeast Asian countries but has been seriously threatened by pathogen. A novel cell line (TOGB) derived from the brain of golden pompano Trachinotus ovatus was established and characterized in this study. TOGB cell line showed high virus susceptibility, especially grouper nervous necrosis virus (GNNV) and Singapore grouper iridovirus (SGIV). As one of the most devastating viruses in marine fish aquaculture, nervous necrosis virus (NNV) causes high mortality rates exceeding 95% in severe outbreaks. Then, TOGB cell line was a useful tool for propagating viruses and provides a potentially valuable resource for the study of viral pathogenesis, the development of antiviral strategies. The TOGB cell lines showed potential application in environmental monitoring. The extracellular products from Vibrio anguillarum and Vibrio alginolyticus demonstrated cytotoxic effects in TOGB cells. TOGB cells grew most rapidly at 28°C, with an optimal concentration of 10% fetal bovine serum in L-15 medium. TOGB cells were diploid (2N = 54). The transfection efficiencies of TOGB cells were 8.6% at the 15th passage and 64.8% at the 45th passage, indica...Continue Reading

References

Oct 31, 1998·Applied and Environmental Microbiology·M C BalebonaJ J Borrego
Mar 1, 2003·Diseases of Aquatic Organisms·Q W QinT J Lam
Nov 29, 2005·Journal of Virological Methods·X H HuangQ Y Zhang
Sep 17, 2009·Journal of Virological Methods·Hai-Dong XuJiang-Yong Wang
Jul 8, 2010·Fish Physiology and Biochemistry·W S LakraK P Joy

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Citations

Feb 27, 2020·Journal of Fish Diseases·Mingzhu LiuPengfei Li
Sep 17, 2021·Learning & Memory·Felippe E AmorimOlavo B Amaral

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