Establishment and characterization of a novel human myoepithelial cell line and matrix-producing xenograft from a parotid basal cell adenocarcinoma

In Vitro Cellular & Developmental Biology. Animal
M D SternlichtS H Barsky

Abstract

Myoepithelial cells exert important paracrine effects on epithelial morphogenesis and mitogenesis through direct cell-cell interactions and through synthesis of a basement membrane extracellular matrix. To study these effects further, this study established the first immortalized human myoepithelial cell line, HMS-1, and transplantable xenograft, HMS-X, from the rare parotid basal cell adenocarcinoma. The cell line exhibited a fully differentiated myoepithelial phenotype and the xenograft exhibited the rare property of accumulating an abundant extracellular matrix composed of both basement membrane and nonbasement membrane components with the latter predominating. With HMS-1 as a feeder layer, dramatic and specific induction of epithelial morphogenesis (spheroid formation) occurred with selected normal epithelial and primary carcinoma target cells. HMS-1 and HMS-X provide distinct advantages over the conventional murine matrices in existence. They will be invaluable in future studies of human tumor-myoepithelial and matrix interactions important for tumor cell growth, invasion, and metastasis.

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Citations

Jun 30, 2006·Journal of Mammary Gland Biology and Neoplasia·Kornelia Polyak, Min Hu
Jun 30, 2006·Journal of Mammary Gland Biology and Neoplasia·Sanford H Barsky, Nina J Karlin
Apr 25, 2003·Experimental and Molecular Pathology·Sanford H Barsky
Aug 19, 2000·In Vitro Cellular & Developmental Biology. Animal·A G PantschenkoT J Yang
Dec 9, 2000·Biochemical and Biophysical Research Communications·M C LeeS H Barsky
Jul 5, 2008·The American Journal of Pathology·Yi XiaoSanford H Barsky
Jun 8, 2006·Oral Oncology·Vera Cavalcanti de AraújoNey Soares de Araújo
Dec 9, 1997·The Journal of Pathology·S H BarskyS M Love
Jan 1, 1997·Medical Hypotheses·M D Sternlicht, S H Barsky
Mar 8, 2006·Nature Clinical Practice. Oncology·Sanford H Barsky, Nina J Karlin
Dec 21, 2002·American Journal of Veterinary Research·Deborah A GrossmanSanford H Barsky
Jan 8, 2014·Veterinary Surgery : VS·Abigail A LockwoodDavid J Schaeffer

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